Guo Y-J, Ji Q-H, Zhou X-Q, Hu Y-P, Guo L-Y, Jiang H, Zeng J-W
Fruit Tree Research Institute, Zhaoqing University, Guangdong, China.
Genet Mol Res. 2013 Jan 30;12(3):3079-87. doi: 10.4238/2013.January.30.7.
The genetic backgrounds of many Citrus varieties are quite complex. Classifications and phylogenetic relationships of Citrus species have become the focus of researchs. Some conserved genes of chloroplast genome's research have been proven effective in determining the biosources of hybrids and phylogenetic analysis. Thus, we studied variations among the chloroplast trnL gene sequences of 10 Citrus species, including C. nobilis Lour. 'Gonggan'. The amplification results of different trnL target genes and identification of the double-enzyme cut after cloning show that lengths of all trnL sequences were within 895 to 935 bp and a total of 24 variation sites were detected among the 10 material samples. Clustering analysis revealed differences in trnL genes caused by systematic evolution and allowed the determination of variations among Citrus varieties. Variation sites of trnL sequences can be used in the phylogenetic classification and species identification of Citrus, and the results agreed with random amplified polymorphic DNA marker results. C. nobilis Lour. 'Gonggan' is closely associated with the other two varieties in Zhaoqing area, and C. nobilis Lour. 'Gonggan' and C. haniana Hort. ex Tseng 'Sihuihanggan' can be classified into the same category. C. nobilis Lour. 'Gonggan' as a natural hybrid is probably a hybrid with C. haniana Hort. ex Tseng 'Sihuihanggan' as its female parent.
许多柑橘品种的遗传背景相当复杂。柑橘属物种的分类和系统发育关系已成为研究的焦点。叶绿体基因组的一些保守基因在确定杂种生物来源和系统发育分析方面已被证明是有效的。因此，我们研究了包括贡柑（Citrus nobilis Lour. 'Gonggan'）在内的10个柑橘品种叶绿体trnL基因序列的变异情况。不同trnL靶基因的扩增结果以及克隆后双酶切鉴定表明，所有trnL序列长度在895至935 bp之间，在10个材料样本中共检测到24个变异位点。聚类分析揭示了由系统进化导致的trnL基因差异，并能够确定柑橘品种间的变异。trnL序列的变异位点可用于柑橘的系统发育分类和物种鉴定，结果与随机扩增多态性DNA标记结果一致。贡柑与肇庆地区的另外两个品种密切相关，贡柑和四会行柑（Citrus haniana Hort. ex Tseng 'Sihuihanggan'）可归为同一类。贡柑作为一个天然杂种，可能是以四会行柑为母本的杂种。
