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测量兽医和人用即时血糖仪与实验室血液分析仪在伊斯帕尼奥拉亚马逊鹦鹉(Amazona ventralis)中的一致性水平。

Measuring the level of agreement between a veterinary and a human point-of-care glucometer and a laboratory blood analyzer in Hispaniolan Amazon parrots (Amazona ventralis).

作者信息

Acierno Mark J, Schnellbacher Rodney, Tully Thomas N

机构信息

School of Veterinary Medicine, Louisiana State University, Skip Bertman Dr, Baton Rouge, LA 70810, USA.

出版信息

J Avian Med Surg. 2012 Dec;26(4):221-4. doi: 10.1647/2011-038R1.1.

Abstract

Although abnormalities in blood glucose concentrations in avian species are not as common as they are in mammals, the inability to provide point-of-care glucose measurement likely results in underreporting and missed treatment opportunities. A veterinary glucometer that uses different optimization codes for specific groups of animals has been produced. To obtain data for a psittacine bird-specific optimization code, as well as to calculate agreement between the veterinary glucometer, a standard human glucometer, and a laboratory analyzer, blood samples were obtained from 25 Hispaniolan Amazon parrots (Amazona ventralis) in a 2-phase study. In the initial phase, blood samples were obtained from 20 parrots twice at a 2-week interval. For each sample, the packed cell volume was determined, and the blood glucose concentration was measured by the veterinary glucometer. The rest of each sample was placed into a lithium heparin microtainer tube and centrifuged, and plasma was removed and frozen at -30 degrees C. Within 5 days, tubes were thawed, and blood glucose concentrations were measured with a laboratory analyzer. The data from both procedures were used to develop a psittacine bird-specific code. For the second phase of the study, the same procedure was repeated twice at a 2-week interval in 25 birds to determine agreement between the veterinary glucometer, a standard human glucometer, and a laboratory analyzer. Neither glucometer was in good agreement with the laboratory analyzer (veterinary glucometer bias, 9.0; level of agreement, -38.1 to 56.2; standard glucometer bias, 69.4; level of agreement -17.8 to 156.7). Based on these results, the use of handheld glucometers in the diagnostic testing of Hispaniolan Amazon parrots and other psittacine birds cannot be recommended.

摘要

尽管鸟类血糖浓度异常不像哺乳动物那样常见,但无法进行即时血糖检测可能导致报告不足和错过治疗机会。现已生产出一种针对特定动物群体使用不同优化代码的兽用血糖仪。为了获取针对吸蜜鹦鹉的优化代码数据,并计算兽用血糖仪、标准家用血糖仪和实验室分析仪之间的一致性,在一项分两阶段的研究中,从25只伊斯帕尼奥拉亚马逊鹦鹉(Amazona ventralis)采集了血样。在初始阶段,每隔两周从20只鹦鹉身上采集两次血样。对于每个样本,测定血细胞比容,并使用兽用血糖仪测量血糖浓度。每个样本的其余部分放入含锂肝素的微量采血管中离心,分离出血浆并在-30℃冷冻。在5天内,将试管解冻,并用实验室分析仪测量血糖浓度。来自这两个程序的数据用于开发针对吸蜜鹦鹉的代码。在研究的第二阶段,对25只鸟每隔两周重复相同程序两次,以确定兽用血糖仪、标准家用血糖仪和实验室分析仪之间的一致性。两种血糖仪与实验室分析仪的一致性均不佳(兽用血糖仪偏差为9.0;一致性水平为-38.1至56.2;标准血糖仪偏差为69.4;一致性水平为-17.8至156.7)。基于这些结果,不建议在伊斯帕尼奥拉亚马逊鹦鹉和其他吸蜜鹦鹉的诊断检测中使用手持式血糖仪。

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