Division of Industrial Food Research, National Food Institute (DTU-Food), Technical University of Denmark, B. 221, Søltofts Plads, DK-2800 Kgs, Lyngby, Denmark.
Food Chem. 2013 Jun 1;138(2-3):1670-81. doi: 10.1016/j.foodchem.2012.10.078. Epub 2012 Nov 15.
Water and ethanolic extracts of 16 species of seaweeds collected along the Danish coasts were screened for antioxidant activities using four in vitro antioxidant assays (2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, reducing power, ferrous ion-chelating and liposome model system). Furthermore their effectiveness in retarding lipid peroxidation in fish oil was evaluated by an accelerated stability test. Significant differences were observed in total and individual phenolic content and the antioxidant activities of seaweed species evaluated. Ethanol was more efficient for polyphenol extraction than water. Polysiphonia fucoides and all the Fucus species tested showed highest radical scavenging activity, reducing power, inhibition of oxidation in liposome model system and in fish oil and were high in phenolic content. These seaweeds could be potential rich sources of natural antioxidants for protection of foods against oxidation. In general, the various antioxidative assays correlated well with the total phenolic content, indicating that algal polyphenols are active components in these extracts. However, in some of the antioxidative assays some species with low total phenolic content also showed good antioxidative effects indicating that some other co-extracted active compounds such as pigments and tocopherols in ethanolic extracts and sulphated polysaccharides, proteins or peptides in water extracts may also contribute to the overall antioxidant properties and this needs further investigation.
沿丹麦海岸采集的 16 种海藻的水提物和醇提物，用四种体外抗氧化测定法（2,2-二苯基-1-苦基肼自由基清除活性、还原力、亚铁离子螯合和脂质体模型系统）进行抗氧化活性筛选。此外，通过加速稳定性试验评估它们在延缓鱼油脂质过氧化中的效果。在所评估的海藻物种中，观察到总酚和个体酚含量以及抗氧化活性存在显著差异。与水相比，乙醇更有利于多酚的提取。裙带菜和所有测试的石莼属物种表现出最高的自由基清除活性、还原力、抑制脂质体模型系统和鱼油中的氧化作用，且酚类含量较高。这些海藻可能是天然抗氧化剂的潜在丰富来源，可用于保护食品免受氧化。总的来说，各种抗氧化测定法与总酚含量相关性良好，表明藻类多酚是这些提取物中的活性成分。然而，在一些抗氧化测定法中，一些总酚含量较低的物种也表现出良好的抗氧化效果，这表明一些其他共提取的活性化合物，如醇提物中的色素和生育酚以及水提物中的硫酸化多糖、蛋白质或肽，也可能对整体抗氧化性能有贡献，这需要进一步研究。
