Alencar Daniel B de, Silva Suzete R da, Pires-Cavalcante Kelma M S, Lima Rebeca L de, Pereira Júnior Francisco N, Sousa Márcia B de, Viana Francisco A, Nagano Celso S, Nascimento Kyria S do, Cavada Benildo S, Sampaio Alexandre H, Saker-Sampaio Silvana
Laboratorio de Produtos Naturais Marinhos, Centro de Ciencias Agrarias, Departamento de Engenharia de Pesca, Universidade Federal do Ceara, Fortaleza, CE, Brasil.
Laboratorio de Biotecnologia Marinha, Centro de Ciencias Agrarias/, Departamento de Engenharia de Pesca, Universidade Federal do Ceara, Fortaleza, CE, Brasil.
An Acad Bras Cienc. 2014 Mar;86(1):251-63. doi: 10.1590/0001-37652014116312.
Natural antioxidants found in marine macroalgae are bioactive compounds known to play an important role in the prevention of diseases associated with aging cells protecting them against the oxidative damage. The purpose of this study was to evaluate the antioxidant and cytotoxic activity of ethanolic extracts of two species of red seaweeds, Amansia multifida and Meristiella echinocarpa. In vitro antioxidant activity was determined by DPPH radical scavenging assay, ferric-reducing antioxidant power (FRAP) assay, ferrous ion chelating (FIC) assay, β-carotene bleaching (BCB) assay and total phenolic content (TPC) quantification. Cytotoxicity was evaluated with the brine shrimp Artemia sp. lethality test. The TPC values observed in the present study indicated that both species A. multifida and M. echinocarpa are rich in phenolic compounds, reaching values of 45.40 and 28.46 mg gallic acid equivalent (GAE) g-1 of ethanolic extract, respectively. DPPH radical scavenging and ferrous ion chelating showed values of 60% and 17%, respectively. Both seaweed extracts inhibited β-carotene oxidation by approximately 40%. None of the algal extracts were potentially cytotoxic. The results have showed that extracts of both species of marine red algae exhibit antioxidant potential and low toxicity. They are sources of natural antioxidant compounds.
在海洋大型藻类中发现的天然抗氧化剂是已知的生物活性化合物，在预防与衰老细胞相关的疾病中发挥重要作用，保护它们免受氧化损伤。本研究的目的是评估两种红藻——多裂阿曼藻（Amansia multifida）和棘果梅里藻（Meristiella echinocarpa）乙醇提取物的抗氧化和细胞毒性活性。通过DPPH自由基清除试验、铁还原抗氧化能力（FRAP）试验、亚铁离子螯合（FIC）试验、β-胡萝卜素漂白（BCB）试验和总酚含量（TPC）定量来测定体外抗氧化活性。用卤虫（Artemia sp.）致死试验评估细胞毒性。本研究中观察到的TPC值表明，多裂阿曼藻和棘果梅里藻这两个物种都富含酚类化合物，乙醇提取物的TPC值分别达到45.40和28.46毫克没食子酸当量（GAE）/克。DPPH自由基清除率和亚铁离子螯合率分别为60%和17%。两种海藻提取物均能抑制约40%的β-胡萝卜素氧化。没有一种藻类提取物具有潜在的细胞毒性。结果表明，这两种海洋红藻的提取物均具有抗氧化潜力且毒性较低。它们都是天然抗氧化化合物的来源。
