Kazan Research Center, Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, PO Box 30, Kazan, 420111, Russia.
World J Microbiol Biotechnol. 2013 Jul;29(7):1189-96. doi: 10.1007/s11274-013-1281-9. Epub 2013 Feb 15.
A new type of plant-derived signal molecules that activate extracellular pectate lyase activity in phytopathogenic bacterium Pectobacterium atrosepticum SCRI1043 was revealed. These compounds were characterized and partially purified by means of several approaches including RT-PCR analysis, luminescence bioassay and HPLC fractionation. They were smaller than 1 kDa, thermoresistant, nonproteinaceous, hydrophilic, and slightly negatively charged molecules. Using gene expression analysis and bacterial biosensor assay the mode of activity of revealed compounds was studied. The possibility of their action through quorum sensing- and KdgR-mediated pathways was analyzed.
一种新型的植物来源的信号分子被揭示，它可以激活植物病原菌果胶酸裂解酶在果胶杆菌 SCRI1043 的细胞外活性。这些化合物通过包括 RT-PCR 分析、发光生物测定和 HPLC 分级分离在内的几种方法进行了表征和部分纯化。它们的分子量小于 1 kDa，耐热，非蛋白，亲水性，略带负电荷。通过基因表达分析和细菌生物传感器测定，研究了所揭示化合物的作用模式。分析了它们通过群体感应和 KdgR 介导的途径作用的可能性。
