Scherk Jordan R, Brainard Benjamin M, Collicutt Nancy B, Bush Sharon E, Almy Frederick S, Koenig Amie
Department of Small Animal Medicine and Surgery, University of Georgia College of Veterinary Medicine, Athens, GA 30602, USA.
J Vet Diagn Invest. 2013 Mar;25(2):219-25. doi: 10.1177/1040638713477644. Epub 2013 Feb 15.
Ethylene glycol (EG) toxicity is commonly encountered in dogs and cats. The purpose of the current study was to determine if the Catachem test kit (Catachem Inc., Oxford, Connecticut) could precisely and accurately detect the presence of EG added to serum and plasma from 6 dogs and 4 cats. Serum and plasma samples were spiked at various concentrations of EG (0, 20, 60, and 100 mg/dl) and analyzed using the Catachem kit. Twenty randomly selected samples were also submitted for gas chromatography-mass spectroscopy (GC-MS) analysis of EG concentration, which was considered the gold standard. Inter- and intra-assay coefficients of variation (CVs) were calculated. Bland-Altman analysis was performed to compare the Catachem results to the GC-MS analyses. Analysis of serum samples showed a bias of 8.48 mg/dl (95% limits of agreement: 17.8 to -0.9 mg/dl) while spiked plasma samples had a bias of 7.32 mg/dl (18.1 to -3.5 mg/dl). Intra-assay CV was 0.7%. Interassay CV ranged from 1.2% to 2.0%. For all samples, the Catachem kit read higher than GC-MS values and slightly overestimated in vitro concentrations. The Catachem test kit is an accurate quantitative test for EG in dogs and cats that may aid in timely recognition of EG exposure. Because of the positive bias in all samples, some pets may receive treatment unnecessarily. However, animals with blood EG concentrations at or above the published lethal serum or plasma concentration will be readily identified so that treatment may be initiated.
乙二醇（EG）中毒在犬猫中较为常见。本研究的目的是确定Catachem检测试剂盒（Catachem公司，牛津，康涅狄格州）能否精确且准确地检测出添加到6只犬和4只猫的血清及血浆中的EG。血清和血浆样本添加了不同浓度的EG（0、20、60和100mg/dl），并使用Catachem试剂盒进行分析。还随机选取了20个样本进行EG浓度的气相色谱 - 质谱联用（GC - MS）分析，GC - MS分析被视为金标准。计算了批内和批间变异系数（CV）。进行Bland - Altman分析以比较Catachem检测结果与GC - MS分析结果。血清样本分析显示偏差为8.48mg/dl（95%一致性界限：17.8至 - 0.9mg/dl），而添加EG的血浆样本偏差为7.32mg/dl（18.1至 - 3.5mg/dl）。批内CV为0.7%。批间CV范围为1.2%至2.0%。对于所有样本，Catachem试剂盒的读数高于GC - MS值，且在体外浓度上略有高估。Catachem检测试剂盒是一种用于犬猫EG检测的准确定量检测方法，有助于及时识别EG暴露情况。由于所有样本均存在正偏差，一些宠物可能会接受不必要的治疗。然而，血液EG浓度达到或高于已公布的致死血清或血浆浓度的动物将很容易被识别出来，从而可以启动治疗。
