Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
J Sci Food Agric. 2013 Jul;93(9):2315-22. doi: 10.1002/jsfa.6045. Epub 2013 Feb 19.
Since tomato is an important food component, it is imperative to enhance its yield against the activities of many devastating fungal pathogens such as Alternaria alternata. The exploitation of plant innate resistance by cultivation of resistant varieties is an effective measure in this regard. In the present study, 28 tomato varieties were tested against 32 A. alternata isolates, and representative varieties were further evaluated to determine the extent and basis of their antifungal resistance.
A significant increase (104.7%) in polyphenols was recorded in the resistant variety Dinaar compared with the susceptible variety Red Tara. Dinaar also exhibited 100% enhancement of alkaloids and terpenoids along with a 30.7% increase in cell wall hemicellulose content. Significant differences were found in physical barriers (cellulose, lignin and pectin) of the representative varieties when stained tissue sections were subjected to colorimetric analysis. Similarly, polyphenol oxidase, peroxidase and phenylalanine ammonia lyase showed increases of 78.37, 114.67 and 125.11% respectively in the resistant variety. Higher expression of glucanase genes was evident from native gel analysis, in which not only the number of isozymes but also the quantity of individual isozymes was significantly increased.
The resistant variety Dinaar had strong antifungal resistance and can therefore be recommended as suitable for cultivation in the agricultural system of Pakistan.
由于番茄是一种重要的食物成分，因此必须增强其产量，以抵御Alternaria alternata 等许多破坏性真菌病原体的侵害。通过培育抗性品种利用植物固有抗性是这方面的有效措施。在本研究中，对 28 个番茄品种进行了 32 个 A. alternata 分离株的测试，并进一步评估了代表性品种，以确定其抗真菌抗性的程度和基础。
与易感品种 Red Tara 相比，抗性品种 Dinaar 的多酚含量显著增加（104.7%）。Dinaar 还表现出生物碱和萜烯的 100%增强，以及细胞壁半纤维素含量增加 30.7%。对代表品种的组织切片进行比色分析时，发现物理屏障（纤维素、木质素和果胶）存在显著差异。同样，多酚氧化酶、过氧化物酶和苯丙氨酸解氨酶在抗性品种中的分别增加了 78.37%、114.67%和 125.11%。从天然凝胶分析中可以明显看出葡聚糖酶基因的高表达，其中不仅同工酶的数量而且单个同工酶的数量都显著增加。
抗性品种 Dinaar 具有很强的抗真菌能力，因此可以推荐在巴基斯坦农业系统中种植。
