Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 108, 3584 CM Utrecht, The Netherlands.
Vet J. 2013 Aug;197(2):192-7. doi: 10.1016/j.tvjl.2013.01.021. Epub 2013 Feb 18.
The gene expression profile of 10 primary canine insulinomas was compared with that of their accompanying metastases using microarray analysis and quantitative real time-PCR. Analysis of microarray data revealed 84 genes that were differentially expressed between primary insulinomas and their metastases, along with 243 genes differentially expressed between a low-metastatic and a high-metastatic subset of primary insulinomas. The genes differently expressed between primary insulinomas and their metastases clustered together in nine signalling pathways. Comparing the low-metastatic to the high-metastatic subset of primary insulinomas, 26 pathways appeared to be significantly influenced. The acinar enzymes pancreatic lipase (PNLIP) and chymotrypsinogen B1 (CTRB1) were amongst the most down-regulated genes in the malignant group of primary insulinomas and in metastases. Immunofluorescence demonstrated co-localisation of insulin and PNLIP in tumour cells. Different subsets of canine insulinomas can be identified on the basis of their gene expression profile. Canine insulinomas appear to contain amphicrine cells, which exhibit both endocrine and exocrine cell features.
使用微阵列分析和实时定量 PCR 比较了 10 个原发性犬胰岛素瘤与其伴随转移瘤的基因表达谱。微阵列数据分析显示，原发性胰岛素瘤与其转移瘤之间有 84 个基因差异表达，低转移和高转移亚组原发性胰岛素瘤之间有 243 个基因差异表达。在 9 个信号通路中，原发性胰岛素瘤与其转移瘤之间差异表达的基因聚集在一起。将低转移组与高转移组原发性胰岛素瘤进行比较，有 26 个通路似乎受到显著影响。在恶性原发性胰岛素瘤和转移瘤中，胰腺脂肪酶 (PNLIP) 和糜蛋白酶原 B1 (CTRB1) 等消化酶基因表达下调最为明显。免疫荧光显示肿瘤细胞中胰岛素和 PNLIP 共定位。基于基因表达谱可以识别不同亚组的犬胰岛素瘤。犬胰岛素瘤似乎含有具有内分泌和外分泌细胞特征的两性细胞。
