Vilnius University, Department of General Physics and Spectroscopy, Sauletekio Avenue 9, LT-10222 Vilnius, Lithuania.
J Biomed Opt. 2013 Feb;18(2):27011. doi: 10.1117/1.JBO.18.2.027011.
Results of the structural analysis of urinary sediments by means of infrared spectral microscopy are presented. The results are in good agreement with the results of standard optical microscopy in the case of single-component and crystalline urinary sediments. It is found that for noncrystalline or multicomponent sediments, the suggested spectroscopic method is superior to optical microscopy. The chemical structure of sediments of any molecular origin can be elucidated by this spectroscopic method. The method is sensitive enough to identify solid particles of drugs present in urine. Sulfamethoxazole and traces of other medicines are revealed in this study among the other sediments. We also show that a rather good correlation exists between the type of urinary sediments and the renal stones removed from the same patient. Spectroscopic studies of urinary stones and corresponding sediments from 76 patients suffering from renal stone disease reveal that in 73% of cases such correlation exists. This finding is a strong argument for the use of infrared spectral microscopy to prevent kidney stone disease because stones can be found in an early stage of formation by using the nonintrusive spectroscopic investigation of urinary sediments. Some medical recommendations concerning the overdosing of certain pharmaceuticals can also be derived from the spectroscopic studies of urinary sediments.
介绍了利用红外光谱显微镜对尿沉淀物进行结构分析的结果。在单一组分和结晶性尿沉淀物的情况下，该结果与标准光学显微镜的结果非常吻合。对于非晶态或多组分沉淀物，建议的光谱方法优于光学显微镜。通过这种光谱方法可以阐明任何分子来源的沉淀物的化学结构。该方法足够灵敏，可以识别尿液中存在的固体药物颗粒。在本研究中，除了其他沉淀物外，还发现了磺胺甲恶唑和其他药物的痕迹。我们还表明，尿沉淀物的类型与从同一患者中取出的肾结石之间存在相当好的相关性。对 76 名患有肾结石病的患者的肾结石和相应沉淀物进行的光谱研究表明，在 73％的情况下存在这种相关性。这一发现强烈支持使用红外光谱显微镜来预防肾结石病，因为通过对尿沉淀物进行非侵入性光谱研究，可以在结石形成的早期阶段发现结石。从尿沉淀物的光谱研究中还可以得出一些关于某些药物过量使用的医学建议。
