Department of Chemical and Petroleum Engineering, Sharif University of Technology, PO Box 11155-9465, Azadi Ave., Tehran, Iran.
Ecotoxicol Environ Saf. 2013 May;91:117-21. doi: 10.1016/j.ecoenv.2013.01.015. Epub 2013 Feb 20.
This paper presents a new method for spectrophotometirc detection of sulfide applying fungal peroxidase immobilized on sodium alginate. The sensing scheme was based on decrease of the absorbance of the orange compound, purpurogallin produced from pyrogallol and H2O2 as substrates, due to the inhibition of peroxidase by sulfide. Absorbance of purpurogallin was detected at 420nm by using a spectrophotometer. The proposed method could successfully detect the sulfide in the concentration range of 0.6-7.0μM with a detection limit of 0.4μM. The kinetic parameters of Michaelis-Menten with and without sulfide were also calculated. Possible inhibition mechanism of peroxidase by sulfide was deduced according to the variation of parameters and uncompetitive mechanism was observed with respect to hydrogen peroxide. The current method provides an easy to use method for sulfide detection in water samples.
本文提出了一种新的应用于基于固定化在海藻酸钠上的真菌过氧化物酶的分光光度法检测硫化物的方法。该传感方案基于由于硫化物抑制过氧化物酶，导致橙色化合物、邻苯三酚和 H2O2 作为底物生成的没食子紫因的吸光度降低。通过分光光度计在 420nm 处检测到没食子紫因的吸光度。该方法可以成功地在 0.6-7.0μM 的浓度范围内检测硫化物，检测限为 0.4μM。还计算了有和没有硫化物时的米氏常数动力学参数。根据参数的变化，推断了硫化物对过氧化物酶的可能抑制机制，观察到了对过氧化氢的非竞争性机制。该方法为水样中的硫化物检测提供了一种简单易用的方法。
