Fan Jun, Li Ruiping, Xu Pingping, Di Junwei, Tu Yifeng, Yan Jilin
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou College of Chemistry, Chemical Engineering and Materials Science, Soochow University.
Anal Sci. 2014;30(4):457-62. doi: 10.2116/analsci.30.457.
In this work, we synthesized a trypsin-stabilized fluorescent gold nanocluster. It was found that sulfide interacted with the nanocluster, which could result in significant fluorescence quenching. With this quenching effect, a fluorescence sulfide sensor was developed. This sensor responded linearly to sulfide in the range of 50 nM to 8 μM, and was capable of detecting sulfide as low as 5.5 nM. This provided a facile and sensitive scheme for sulfide analysis; the mechanism of the sensor was also provided. The sensor was then tested for real sample analysis, and good recoveries were obtained. Furthermore, persulfate was found to be effective to remove the quenching of sulfide, and this interaction was adopted for an indirect analysis of persulfate.
在本工作中，我们合成了一种胰蛋白酶稳定的荧光金纳米簇。发现硫化物与该纳米簇相互作用，这可能导致显著的荧光猝灭。基于这种猝灭效应，开发了一种荧光硫化物传感器。该传感器对硫化物在50 nM至8 μM范围内呈线性响应，并且能够检测低至5.5 nM的硫化物。这为硫化物分析提供了一种简便且灵敏的方案；同时还给出了该传感器的作用机制。然后对该传感器进行实际样品分析测试，并获得了良好的回收率。此外，发现过硫酸盐可有效消除硫化物的猝灭作用，并且这种相互作用被用于过硫酸盐的间接分析。
