Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Cell Immunol. 2013 Jan;281(1):37-43. doi: 10.1016/j.cellimm.2013.01.008. Epub 2013 Feb 4.
Two hundred and fifty 11-day-old chickens were randomly assigned into 5 groups and except normal control group injected with cyclophosphamide once a day for 3 successive days. At day-14-old, all chickens were vaccinated with Newcastle disease vaccine. At the same time of the first vaccination, the chickens in three experimental groups were injected respectively with epimedium polysaccharide-propolis flavone immunopotentiator (EPI) at three dosages, once a day for 3 successive days. On days 7, 14, 21 and 28 after the first vaccination, the serum antibody titer and IgG, IgM, IFN-γ and IL-6 concentrations, peripheral lymphocyte proliferation, including immune organ index on day 28, were measured. The results demonstrated that EPI at high and medium doses could significantly enhance antibody titer and IgG, IgM, IFN-γ and IL-6 concentrations, promote lymphocyte proliferation and enlarge immune organ index as compared with model control group. This indicated that EPI could effectively resist the immunosuppression.
250 只 11 日龄雏鸡随机分为 5 组，除正常对照组外，其余各组连续 3 天每天腹腔注射环磷酰胺 1 次。于 14 日龄时，所有雏鸡均用新城疫疫苗进行首免。首免的同时，3 个实验组雏鸡分别按不同剂量连续 3 天每天腹腔注射淫羊藿多糖-蜂胶黄酮免疫增强剂（EPI）。首免后第 7、14、21 和 28 天，检测血清抗体效价和 IgG、IgM、IFN-γ和 IL-6 浓度、外周血淋巴细胞增殖情况，以及 28 天的免疫器官指数。结果表明，高、中剂量 EPI 可显著提高抗体效价和 IgG、IgM、IFN-γ和 IL-6 浓度，促进淋巴细胞增殖，增大免疫器官指数，与模型对照组相比差异显著。表明 EPI 能有效抵抗免疫抑制。
