Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Num. 1 Weigang East Zhongshan Road, Nanjing 210095, PR China.
Int J Biol Macromol. 2010 Aug 1;47(2):87-92. doi: 10.1016/j.ijbiomac.2010.05.017. Epub 2010 Jun 1.
Four prescriptions, epimedium flavone plus propolis flavone (EF-PF), epimedium flavone plus propolis extracts (EF-PE), epimedium polysaccharide plus propolis flavone (EP-PF) and epimedium polysaccharide plus propolis extracts (EP-PE), were prepared and their immune-enhancing effects were compared. In test in vitro, the effects of them on chicken peripheral lymphocyte proliferation were determined by MTT method. The results showed that EP-PF group presented the highest stimulating index at most concentrations. In immune test, 300 14-day-old chickens were randomly divided into six groups and vaccinated with ND vaccine except for blank control (BC) group, re-challenged at 28 days of age. At the same time of the first vaccination, the chickens in four experimental groups were injected, respectively, with four prescriptions. The changes of the lymphocyte proliferation and antibody titer were determined. On day 28 after the first vaccination, the chickens except for BC group were challenged with NDV, the immune protective effect was observed. The results displayed that in EP-PF group, the antibody titers, lymphocyte proliferation and protective rate were the highest, the morbidity and mortality were the lowest. In dose test, 14-day-old chickens were randomly divided into five groups. The treatment and determinations were the same as the immune test except that the chickens in experimental groups were injected, respectively, with high, medium and low doses of EP-PF. The results revealed that in medium dose group, the antibody titers, lymphocyte proliferation and protective rate were the highest, the morbidity and mortality were the lowest. These results indicated that EP and PF possessed synergistically immune enhancement, EP-PF had the best efficacy, especially at medium dose, and would be expected to exploit into a new-type immunopotentiator.
四种配方,淫羊藿黄酮加蜂胶黄酮(EF-PF)、淫羊藿黄酮加蜂胶提取物(EF-PE)、淫羊藿多糖加蜂胶黄酮(EP-PF)和淫羊藿多糖加蜂胶提取物(EP-PE),被制备并比较其免疫增强作用。在体外试验中,通过 MTT 法测定它们对鸡外周血淋巴细胞增殖的影响。结果表明,在大多数浓度下,EP-PF 组的刺激指数最高。在免疫试验中,将 300 只 14 日龄鸡随机分为 6 组,除空白对照组(BC)外,均用 ND 疫苗免疫,28 日龄再次攻毒。同时,在第一次免疫时,分别给四个实验组注射四种配方。测定淋巴细胞增殖和抗体效价的变化。在第一次免疫后第 28 天,除 BC 组外,所有鸡均用 NDV 攻毒,观察免疫保护效果。结果显示,在 EP-PF 组中,抗体滴度、淋巴细胞增殖和保护率最高,发病率和死亡率最低。在剂量试验中,将 14 日龄鸡随机分为五组。处理和测定与免疫试验相同,只是实验组鸡分别注射高、中、低剂量的 EP-PF。结果表明,在中剂量组中,抗体滴度、淋巴细胞增殖和保护率最高,发病率和死亡率最低。这些结果表明 EP 和 PF 具有协同的免疫增强作用,EP-PF 效果最佳,尤其是在中剂量时,有望开发成新型免疫增强剂。