Biosystems Technology, Wildau University of Applied Sciences, Bahnhofstrasse 1, 15745 Wildau, Germany.
Anal Bioanal Chem. 2013 Apr;405(11):3739-52. doi: 10.1007/s00216-013-6789-1. Epub 2013 Feb 26.
The review covers recent developments in which quantum dots (QDs) are combined with electrodes for detection of analytes. Special focus will be on the generation of photocurrents and the possibility of spatially resolved, light-directed analysis. Different modes for combining biochemical reactions with QDs will be discussed. Other applications involve the use of QDs as labels in binding analysis. Different methods have been developed for read-out. In addition to photocurrent analysis, voltammetric detection of metals and electrochemiluminescence (ECL) can be used. In the latter, light is the sensor signal. ECL-based systems combine the advantage of very sensitive analytical detection with rather simple instrumentation.
本文综述了近年来将量子点 (QD) 与电极结合用于分析物检测的最新进展。特别关注的是光电流的产生和空间分辨、光导向分析的可能性。本文还讨论了将生化反应与 QD 结合的不同模式。其他应用包括将 QD 用作结合分析中的标记物。已经开发出不同的方法来进行读出。除了光电流分析之外，还可以使用金属的伏安检测和电化学发光 (ECL)。在后一种情况下，光就是传感器信号。基于 ECL 的系统结合了非常灵敏的分析检测的优势和相当简单的仪器。
