脂肪酶酵母利用油菜籽油生产 α-酮戊二酸。

α-Ketoglutaric acid production from rapeseed oil by Yarrowia lipolytica yeast.

机构信息

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, Moscow Region 142290, Russia.

出版信息

Appl Microbiol Biotechnol. 2013 Jun;97(12):5517-25. doi: 10.1007/s00253-013-4772-6. Epub 2013 Feb 26.

DOI:10.1007/s00253-013-4772-6

PMID:23435985

Abstract

The possibility of using rapeseed oil as a carbon source for microbiological production of α-ketoglutaric acid (KGA) has been studied. Acid formation on the selective media has been tested in 26 strains of Yarrowia lipolytica yeast, and the strain Y. lipolytica VKM Y-2412 was selected as a prospective producer of KGA from rapeseed oil. KGA production by the selected strain was studied in dependence on thiamine concentration, medium pH, temperature, aeration, and concentration of oil. Under optimal conditions (thiamine concentration of 0.063 μg g cells(-1), pH 3.5, 30 °C, high dissolved oxygen concentration (pO2) of 50 % (of air saturation), and oil concentration in a range from 20 to 60 g l(-1)), Y. lipolytica VKM Y-2412 produced up to 102.5 g l(-1) of KGA with the mass yield coefficient of 0.95 g g(-1) and the volumetric KGA productivity (QKGA) of 0.8 g l(-1) h(-1).

摘要

已经研究了将菜籽油用作微生物生产α-酮戊二酸（KGA）的碳源的可能性。在 26 株解脂耶氏酵母的选择性培养基上测试了酸的形成，选择解脂耶氏酵母 VKM Y-2412 菌株作为从菜籽油生产 KGA 的有前途的生产者。研究了所选菌株在依赖于硫胺素浓度、培养基 pH 值、温度、通气和油浓度的条件下生产 KGA。在最佳条件下（硫胺素浓度为 0.063 μg g 细胞(-1)，pH 值 3.5，30°C，高溶解氧浓度（pO2）为 50%（空气饱和度），油浓度在 20 至 60 g l(-1)范围内），解脂耶氏酵母 VKM Y-2412 生产了高达 102.5 g l(-1)的 KGA，产率系数为 0.95 g g(-1)，比 KGA 体积生产率（QKGA）为 0.8 g l(-1) h(-1)。