基于质谱的方法研究蛋白质结构与动态。
Mass spectrometry-based methods to study protein architecture and dynamics.
机构信息
Department of Chemistry, University of Massachusetts-Amherst, Amherst, Massachusetts, USA.
出版信息
Protein Sci. 2013 May;22(5):530-44. doi: 10.1002/pro.2238. Epub 2013 Mar 26.
Abstract
Mass spectrometry is now an indispensable tool in the armamentarium of molecular biophysics, where it is used for tasks ranging from protein sequencing and mapping of post-translational modifications to studies of higher order structure, conformational dynamics, and interactions of proteins with small molecule ligands and other biopolymers. This mini-review highlights several popular mass spectrometry-based tools that are now commonly used for structural studies of proteins beyond their covalent structure with a particular emphasis on hydrogen exchange and direct electrospray ionization mass spectrometry.
摘要
质谱现在是分子生物物理学中不可或缺的工具，它可用于从蛋白质测序和翻译后修饰图谱绘制到高级结构、构象动力学以及蛋白质与小分子配体和其他生物聚合物相互作用的研究等各种任务。这篇迷你综述突出了几种目前常用于蛋白质结构研究的基于质谱的流行工具，特别强调了氢交换和直接电喷雾电离质谱。
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