能够分解碱木质素的链霉菌 spp. 菌株 F-6 和 F-7 的分离和特性描述。
Isolation and characterization of Streptomyces spp. strains F-6 and F-7 capable of decomposing alkali lignin.
机构信息
School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
出版信息
Environ Technol. 2012 Dec;33(22-24):2603-9. doi: 10.1080/09593330.2012.672473.
Biodegradation and bioconversion of lignin are the result of the combined action of fungi, bacteria and actinomycetes. Through screening from forest soil, two novel isolated actinomycete strains were identified as Streptomyces spp. strains F-6 and F-7 by their morphology, cultural characteristics and high homology to the 16S rRNA gene. Both strains possessed laccase and manganese peroxidase activities. Laccase activity produced by strain F-6 was up to 935.4 U g(-1) dry cell weight. More than 50% of alkali lignin was removed by strains F-6 and F-7 in 12 days of incubation. GC-MS analysis of the biodegraded products showed strain F-6 converted lignin into phenol and broken phenol compounds. The two strains could co-culture with white-rot fungus, and the combined actinonycete-fungus system decomposed alkali lignin effectively.
木质素的生物降解和生物转化是真菌、细菌和放线菌共同作用的结果。通过从森林土壤中筛选，两种新型分离的放线菌菌株 F-6 和 F-7 被鉴定为链霉菌属菌株，其形态、培养特性和 16S rRNA 基因的高度同源性。这两种菌株都具有漆酶和锰过氧化物酶活性。菌株 F-6 产生的漆酶活性高达 935.4 U g(-1)干细胞重量。在 12 天的培养过程中，菌株 F-6 和 F-7 去除了超过 50%的碱性木质素。生物降解产物的 GC-MS 分析表明，菌株 F-6 将木质素转化为苯酚和断裂的苯酚化合物。这两种菌株可以与白腐菌共培养，联合放线菌-真菌系统有效地分解碱性木质素。
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