Division of Applied Sciences, College of Environmental Technology, Graduate School of Engineering, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Hokkaido, Japan.
Department of Pharmacy, College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Bioresour Technol. 2014;152:429-36. doi: 10.1016/j.biortech.2013.11.032. Epub 2013 Nov 21.
Effective biological pretreatment method for enhancing cellulase performance was investigated. Two alkali lignin-degrading bacteria were isolated from forest soils in Japan and named CS-1 and CS-2. 16S rDNA sequence analysis indicated that CS-1 and CS-2 were Bacillus sp. Strains CS-1 and CS-2 displayed alkali lignin degradation capability. With initial concentrations of 0.05-2.0 g L(-1), at least 61% alkali lignin could be degraded within 48 h. High laccase activities were observed in crude enzyme extracts from the isolated strains. This result indicated that alkali lignin degradation was correlated with laccase activities. Judging from the net yields of sugars after enzymatic hydrolysis, the most effective pretreatment method for enhancing cellulase performance was a two-step processing procedure (pretreatment using Bacillus sp. CS-1 followed by lactic acid bacteria) at 68.6%. These results suggest that the two-step pretreatment procedure is effective at accelerating cellulase performance.
研究了一种有效的生物预处理方法，以提高纤维素酶的性能。从日本森林土壤中分离到两种降解碱木质素的细菌，分别命名为 CS-1 和 CS-2。16S rDNA 序列分析表明，CS-1 和 CS-2 均为芽孢杆菌属。菌株 CS-1 和 CS-2 显示出碱木质素降解能力。在初始浓度为 0.05-2.0 g/L 时，至少 61%的碱木质素可在 48 小时内降解。从分离菌株的粗酶提取物中观察到高漆酶活性。这一结果表明，碱木质素的降解与漆酶活性有关。从酶解后糖的净产量来看，提高纤维素酶性能的最有效预处理方法是两步处理程序（先用芽孢杆菌 CS-1 预处理，再用乳酸菌），达到 68.6%。这些结果表明，两步预处理程序可有效加速纤维素酶的性能。
