Krause U, Beyer J
Dtsch Med Wochenschr. 1975 Feb 7;100(6):238-40. doi: 10.1055/s-0028-1106201.
Insulin preparations available in Germany were tested by polyacrylamide-disc electrophoresis. A large proportion of preparations contained proteins additional to insulin. Insulin purified chromatographically was cleaner than that by crystallization. Insulin purified by ion-exchange chromatography had the highest degree of purity: in addition to the insulin band only deamidation products of insulin were demonstrated. Deamidation occurs both in neutral and acid solvents. These observations indicate that commercial full purification of insulin is possible but not always practised.
德国市场上销售的胰岛素制剂通过聚丙烯酰胺圆盘电泳进行了检测。大部分制剂除胰岛素外还含有其他蛋白质。通过色谱法纯化的胰岛素比通过结晶法纯化的胰岛素更纯净。通过离子交换色谱法纯化的胰岛素纯度最高：除胰岛素条带外，仅显示出胰岛素的脱酰胺产物。脱酰胺反应在中性和酸性溶剂中均会发生。这些观察结果表明，胰岛素的商业全纯化是可行的，但并非总是如此。
