Rusakov Iu I, Bondareva V M
Zh Evol Biokhim Fiziol. 1979 Mar-Apr;15(2):136-40.
Crystalline insulins have been obtained from the insular tissue of the teleosts O. gorbuscha and Th. chalcogramma which is located separately from the exocrine tissue. Some tests to the homogeneity and purity of the preparations obtained were made. Biological activity of insulin as revealed by seizure tests in mice is equal to 20.1 +/- 1.19 IU/mg in O. gorbuscha and 16.9 +/- 1.99 IU/mg in Th. chalcogramma, amounting correspondingly to 83 and 70% of the activity of mammalian standard insulin. The yield of preparations calculated per wet weight of the insular tissue is equal to 2 mg/g in O. gorbuscha and 0.7 mg/g in Th. chalcogramma. It is significantly higher than the yield of mammalian insulins calculated per the weight of the pancreas. This finding may be of some interest with respect to the use of fish as a source of hormonal preparations.
已从与外分泌组织分开的硬骨鱼戈氏油鲱和黄斑海鲶的胰岛组织中获得了结晶胰岛素。对所获得制剂的均一性和纯度进行了一些检测。通过小鼠惊厥试验揭示的胰岛素生物活性在戈氏油鲱中为20.1±1.19 IU/mg，在黄斑海鲶中为16.9±1.99 IU/mg，分别相当于哺乳动物标准胰岛素活性的83%和70%。按胰岛组织湿重计算的制剂产量在戈氏油鲱中为2 mg/g，在黄斑海鲶中为0.7 mg/g。这明显高于按胰腺重量计算的哺乳动物胰岛素产量。这一发现对于将鱼类用作激素制剂来源可能具有一定意义。
