Baheti K G, Shah N, Shaikh S
Department of Pharmaceutical Chemistry, Y. B. Chavan College of Pharmacy, Rauza Baugh, Aurangabad-431 001, India.
Indian J Pharm Sci. 2012 May;74(3):271-4. doi: 10.4103/0250-474X.106076.
A sensitive, accurate, precise and validated ion-pairing reverse-phase liquid chromatographic method for the quantitative determination of atenolol and indapamide in bulk and tablet dosage form was developed. The proposed ion-pairing reverse-phase high performance liquid chromatography method utilises C(18) column with 5 μm, 150×4.6 mm i.d. column and mobile phase consisting of 0.1% w/v solution of octane sulphonic acid, sodium salt and methanol (55:45 v/v), (pH 2.8) and ultraviolet detection at 235 nm. A linearity range of 1-250 μg/ml and 1-25 μg/ml for atenolol and indapamide, respectively, was obtained. The mean recoveries are 100.48 and 99.82% for atenolol and indapamide, respectively. The method was validated as per International Conference on Harmonization guidelines.
建立了一种灵敏、准确、精密且经过验证的离子对反相液相色谱法，用于定量测定原料药和片剂剂型中的阿替洛尔和吲达帕胺。所提出的离子对反相高效液相色谱法使用内径为150×4.6 mm、粒径为5 μm的C(18)柱，流动相由0.1% w/v的辛烷磺酸钠溶液和甲醇（55:45 v/v）组成（pH 2.8），并在235 nm处进行紫外检测。阿替洛尔和吲达帕胺的线性范围分别为1 - 250 μg/ml和1 - 25 μg/ml。阿替洛尔和吲达帕胺的平均回收率分别为100.48%和99.82%。该方法按照国际协调会议指南进行了验证。
