Swedberg S A
Hewlett-Packard Laboratories, Chemical Systems Department, Palo Alto, California 94303.
Anal Biochem. 1990 Feb 15;185(1):51-6. doi: 10.1016/0003-2697(90)90253-6.
Original calculations of over a million theoretical plate efficiency for macromolecular solutes in the open tubular high-performance capillary electrophoresis experiment considered axial diffusion to be the efficiency limiting factor. In practice, interactions of biopolymers, such as proteins, with the capillary wall has had a significant impact on readily achieving high efficiencies for a wide variety of proteins. This paper reports a capillary system in which protein-surface interactions have been minimized, resulting in high efficiencies (greater than or equal to 300,000 theoretical plates). This system allows the analysis of a set of protein standards over a wide pI range at neutral pH and moderate ionic strength. The characterization of the behavior of those protein standards in this capillary system is described.
在开管高效毛细管电泳实验中，最初针对大分子溶质超过一百万理论塔板数效率的计算认为轴向扩散是效率限制因素。实际上，生物聚合物（如蛋白质）与毛细管壁的相互作用对多种蛋白质轻松实现高效分离产生了重大影响。本文报道了一种毛细管系统，其中蛋白质与表面的相互作用已降至最低，从而实现了高效率（大于或等于300,000理论塔板数）。该系统允许在中性pH和中等离子强度下，对一系列宽pI范围内的蛋白质标准品进行分析。文中描述了这些蛋白质标准品在该毛细管系统中的行为特征。
