Lindner H, Helliger W, Dirschlmayer A, Jaquemar M, Puschendorf B
Institute of Medical Chemistry and Biochemistry, Innsbruck, Austria.
Biochem J. 1992 Apr 15;283 ( Pt 2)(Pt 2):467-71. doi: 10.1042/bj2830467.
By using high-performance capillary electrophoresis, we have successfully separated rat liver core histones into several subfractions. Inconvenient interactions of the highly basic proteins with the capillary wall were eliminated by a phosphate buffer system containing 0.03% hydroxyprophylmethylcellulose. Sample amounts of a few nanolitres were analysed within about 20 min. Multiacetylated histones H4 and H3 from induced Friend erythroleukaemic cells prepurified by h.p.l.c. were clearly separated into their non-acetylated and distinct acetylated forms. Our results illustrate that the application of capillary zone electrophoresis on its own or in combination with h.p.l.c. to the analysis of histones provides an important new alternative to traditional gel electrophoreses.
通过使用高效毛细管电泳，我们成功地将大鼠肝脏核心组蛋白分离成几个亚组分。含有0.03%羟丙基甲基纤维素的磷酸盐缓冲系统消除了高碱性蛋白质与毛细管壁之间不便的相互作用。在约20分钟内分析了几纳升的样品量。通过高效液相色谱预纯化的诱导型弗氏红白血病细胞中的多乙酰化组蛋白H4和H3被清晰地分离成其非乙酰化和不同的乙酰化形式。我们的结果表明，毛细管区带电泳单独应用或与高效液相色谱联用用于组蛋白分析，为传统凝胶电泳提供了一种重要的新选择。
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