噬菌体 T4 溶菌酶的正交自旋标记和 Gd(III)-氮氧自由基距离测量。
Orthogonal spin labeling and Gd(III)-nitroxide distance measurements on bacteriophage T4-lysozyme.
机构信息
Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland.
出版信息
J Phys Chem B. 2013 Mar 21;117(11):3145-53. doi: 10.1021/jp401806g. Epub 2013 Mar 8.
Abstract
We present the first example of chemoselective site-specific spin labeling of a monomeric protein with two spectroscopically orthogonal spin labels: a gadolinium(III) chelate complex and a nitroxide radical. A detailed analysis of the performance of two commercially available Gd(III) ligands in the Gd(III)-nitroxide pulse double electron-electron resonance (DEER or PELDOR) experiment is reported. A modification of the flip angle of the pump pulse in the Gd(III)-nitroxide DEER experiment is proposed to optimize sensitivity.
摘要
我们提出了首例利用两种光谱正交的自旋标记物
镧系金属螯合物复合物和氮氧自由基，对单体蛋白进行化学选择性定点自旋标记的实例。详细分析了两种市售的 Gd(III)配体在 Gd(III)-氮氧自由基脉冲双电子-电子共振（DEER 或 PELDOR）实验中的性能。提出了在 Gd(III)-氮氧自由基 DEER 实验中改变泵浦脉冲翻转角以优化灵敏度的方法。
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本文引用的文献
1
Determination of End-to-End Distances in a Series of TEMPO Diradicals of up to 2.8 nm Length with a New Four-Pulse Double Electron Electron Resonance Experiment.
Angew Chem Int Ed Engl. 1998 Nov 2;37(20):2833-2837. doi: 10.1002/(SICI)1521-3773(19981102)37:20<2833::AID-ANIE2833>3.0.CO;2-7.
2
Multiple Pathway Relaxation Enhancement in the System Composed of Three Paramagnetic Species: Nitroxide Radical-Ln(3+)-O2.
J Phys Chem Lett. 2012 May 17;3(10):1336-40. doi: 10.1021/jz300316q. Epub 2012 May 4.
3
Distance measurements on orthogonally spin-labeled membrane spanning WALP23 polypeptides.
J Phys Chem B. 2013 Feb 21;117(7):2061-8. doi: 10.1021/jp311287t. Epub 2013 Feb 8.
4
Trityl radicals: spin labels for nanometer-distance measurements.
Chemistry. 2012 Oct 22;18(43):13580-4. doi: 10.1002/chem.201203014. Epub 2012 Sep 20.
5
Determining α-helical and β-sheet secondary structures via pulsed electron spin resonance spectroscopy.
Biochemistry. 2012 Sep 25;51(38):7417-9. doi: 10.1021/bi3010736. Epub 2012 Sep 14.
6
High sensitivity and versatility of the DEER experiment on nitroxide radical pairs at Q-band frequencies.
Phys Chem Chem Phys. 2012 Aug 14;14(30):10762-73. doi: 10.1039/c2cp41520h. Epub 2012 Jul 3.
7
Distance measurements in Au nanoparticles functionalized with nitroxide radicals and Gd(3+)-DTPA chelate complexes.
Phys Chem Chem Phys. 2012 Aug 14;14(30):10732-46. doi: 10.1039/c2cp40282c. Epub 2012 Jun 28.
8
DEER distance measurements on proteins.
Annu Rev Phys Chem. 2012;63:419-46. doi: 10.1146/annurev-physchem-032511-143716. Epub 2012 Jan 30.
9
Spectroscopic selection of distance measurements in a protein dimer with mixed nitroxide and Gd3+ spin labels.
Phys Chem Chem Phys. 2012 Apr 7;14(13):4355-8. doi: 10.1039/c2cp40219j. Epub 2012 Feb 23.
10
Structure and dynamics of nucleic acids.
Top Curr Chem. 2012;321:159-98. doi: 10.1007/128_2011_300.
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