Department of General Dental Practice and Oral & Maxillofacial Imaging, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
J Periodontal Res. 2013 Oct;48(5):663-70. doi: 10.1111/jre.12062. Epub 2013 Feb 27.
Studies of peri-implant soft tissue on in vivo models are commonly based on histological sections prepared using undecalcified or 'fracture' techniques. These techniques require the cutting or removal of implant during the specimen preparation process. The aim of this study is to explore a new impression technique that does not require any cutting or removal of implant for contour analysis of soft tissue around four types of titanium (Ti) surface roughness using an in vitro three-dimensional oral mucosal model (3D OMM).
The 3D OMM was constructed by co-culturing a keratinocyte cell line TR146 and human oral fibroblasts on to an acellular dermis scaffold. On the fourth day, a Ti disk was placed into the model. Four types of Ti surface topographies, i.e. polished, machined, sandblasted and anodized were tested. After 10 d of culture, the specimens were processed based on undecalcified (ground sectioning), electropolishing and impression techniques for contour analysis of the implant-soft tissue interface.
Under light microscopic examination of the ground and electropolishing sections, it was found that the cell line-based oral mucosa formed a peri-implant-like epithelium attachment on to all four types of Ti surfaces. In contour analysis, the most common contour observed between the cell line-based oral mucosa and Ti surface was at an angle ranging between 45° and 90°.
The in vitro cell line-based 3D OMM formed a peri-implant-like epithelium at the implant-soft tissue interface. The contour of the implant-soft tissue interface for the four types of Ti surface was not significantly different.
在体内模型中研究种植体周围软组织通常基于使用非脱钙或“断裂”技术制备的组织学切片。这些技术在标本制备过程中需要切割或去除植入物。本研究旨在探索一种新的印模技术，该技术不需要切割或去除植入物，即可使用体外三维口腔黏膜模型（3D OMM）分析四种钛（Ti）表面粗糙度周围软组织的轮廓。
通过将角质细胞系 TR146 和人口腔成纤维细胞共培养到脱细胞真皮支架上构建 3D OMM。第四天，将 Ti 圆盘放入模型中。测试了四种 Ti 表面形貌，即抛光、机械加工、喷砂和阳极氧化。培养 10 天后，根据非脱钙（磨片）、电化学抛光和印模技术对标本进行处理，以分析植入物-软组织界面的轮廓。
在磨片和电化学抛光切片的光镜检查下，发现基于细胞系的口腔黏膜在所有四种 Ti 表面上形成了类似于种植体的上皮附着。在轮廓分析中，在细胞系口腔黏膜和 Ti 表面之间观察到的最常见轮廓在 45°和 90°之间的角度范围内。
体外细胞系 3D OMM 在植入物-软组织界面形成类似于种植体的上皮。四种 Ti 表面的植入物-软组织界面的轮廓没有显著差异。
