Razali Masfueh, Ngeow Wei Cheong, Omar Ros Anita, Chai Wen Lin
Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.
Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur 50603, Malaysia.
Biomedicines. 2021 Jan 15;9(1):78. doi: 10.3390/biomedicines9010078.
The presence of epithelial and connective tissue attachment at the peri-implant-soft tissue region has been demonstrated to provide a biological barrier of the alveolar bone from the oral environment. This barrier can be improved via surface modification of implant abutment materials. The effect of photofunctionalization on creating a bioactive surface for the enhancement of the epithelial and connective tissue attachment of zirconia implant abutment's peri-implant mucosal interface using organotypic model has not been investigated. Therefore, this study aimed to evaluate the soft tissue seal around peri-implant mucosa and to understand the effect of photofunctionalization on the abutment materials. Three types of abutment materials were used in this study; yttria-stabilized zirconia (YSZ), alumina-toughened zirconia, and grade 2 commercially pure titanium (CPTi) which were divided into nontreated (N-Tx) and photofunctionalized group (UV-Tx). The three-dimensional peri-implant mucosal model was constructed using primary human gingival keratinocytes and fibroblasts co-cultured on the acellular dermal membrane. The biological seal was determined through the concentration of tritiated water permeating the material-soft tissue interface. The biological seal formed by the soft tissue in the N-Tx group was significantly reduced compared to the UV-treated group ( < 0.001), with YSZ exhibiting the lowest permeability among all materials. Photofunctionalization of implant abutment materials improved the biological seal of the surrounding soft tissue peri-implant interface.
种植体周围软组织区域上皮和结缔组织附着的存在已被证明可为牙槽骨提供一个与口腔环境隔离的生物屏障。通过种植体基台材料的表面改性可以改善这一屏障。尚未研究光功能化对使用器官型模型增强氧化锆种植体基台种植体周围黏膜界面上皮和结缔组织附着的生物活性表面的影响。因此，本研究旨在评估种植体周围黏膜周围的软组织封闭情况，并了解光功能化对基台材料的影响。本研究使用了三种类型的基台材料；氧化钇稳定氧化锆（YSZ）、氧化铝增韧氧化锆和2级商业纯钛（CPTi），它们被分为未处理组（N-Tx）和光功能化组（UV-Tx）。使用在无细胞真皮膜上共培养的原代人牙龈角质形成细胞和成纤维细胞构建三维种植体周围黏膜模型。通过渗透材料-软组织界面的氚标记水的浓度来确定生物封闭情况。与紫外线处理组相比，N-Tx组软组织形成的生物封闭明显降低（<0.001），其中YSZ在所有材料中表现出最低的渗透性。种植体基台材料的光功能化改善了种植体周围软组织界面周围的生物封闭。
