Laboratory Medicine of Yijishan Hospital, Wuhu, Anhui 241001, PR China.
Int J Mol Med. 2013 Apr;31(4):797-802. doi: 10.3892/ijmm.2013.1260. Epub 2013 Jan 30.
microRNAs (miRNAs) play a crucial role in tissue development and the pathology of various diseases. However, the effects and roles of miRNAs in macrophage polarization have yet to be investigated. In this study, we analyzed and compared the miRNA expression profiles of bone marrow-derived macrophages (BMDMs) with two distinct polarizing conditions (classical macrophage activation 'M1' and alternative activation 'M2') using miRNA microarray. In total, 109 miRNAs were differentially expressed between M1 and M2. The differential expression of selected miRNAs was validated by real-time qRT-PCR: miR-181a, miR-155-5p, miR-204-5p and miR-451 were upregulated (fold change >2, P<0.05) and miR-125-5p, miR-146a-3p, miR-143-3p and miR-145-5p were downregulated (fold change <-2, P<0.05) in M1 compared with M2. In conclusion, our study may be useful for exploring the precise roles of miRNAs in macrophage differentiation and polarized activation processes in the future.
microRNAs (miRNAs) 在组织发育和各种疾病的病理学中起着至关重要的作用。然而，miRNAs 在巨噬细胞极化中的作用和功能仍有待研究。在本研究中，我们使用 miRNA 微阵列分析和比较了具有两种不同极化状态（经典巨噬细胞激活 'M1' 和替代激活 'M2'）的骨髓来源巨噬细胞（BMDMs）的 miRNA 表达谱。总共在 M1 和 M2 之间差异表达了 109 个 miRNA。通过实时 qRT-PCR 验证了选定 miRNA 的差异表达：miR-181a、miR-155-5p、miR-204-5p 和 miR-451 上调（倍数变化 >2，P<0.05），而 miR-125-5p、miR-146a-3p、miR-143-3p 和 miR-145-5p 在 M1 中下调（倍数变化 <-2，P<0.05）与 M2 相比。总之，我们的研究可能有助于未来探索 miRNA 在巨噬细胞分化和极化激活过程中的精确作用。
