Zhou Xinhua, Huang Xueping, Huang Zuoping, Wang Zhiqiang, Zhao Tong
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Feb;33(2):235-8.
To explore the effect of CD99 overexpression on the morphology and differentiation-related phenotypes of classical Hodgkin's lymphoma cell line L428 and investigate the role of CD99 gene in Hodgkin/Reed-Sternberg (HRS) cell generation and transformation.
The effect of CD99 overexpression on the cell morphology was detected by HE staining and phalloidin staining. Differentiation-related protein expressions were detected by immunocytochemistry and flow cytometry after stable transfection of CD99 gene in L428 cells.
CD99 overexpression caused a decrease of the cell size and reorganization of the actin cytoskeleton in L428 cells. Upregulation of CD99 led to the loss of classical Hodgkin's lymphoma diagnosis marker CD30 and CD15 and the restoration of the B-cell makers of PAX5, CD19, CD79α, BCL-6, and CD10.
CD99 overexpression leads to redifferentiation of L428 cells towards B cells, suggesting that the loss of B-cell phenotype in classical Hodgkin's lymphoma is very likely a result of down-regulated CD99 expression.
探讨CD99过表达对经典型霍奇金淋巴瘤细胞系L428形态及分化相关表型的影响，研究CD99基因在霍奇金/里德-斯腾伯格（HRS）细胞产生及转化中的作用。
通过苏木精-伊红（HE）染色和鬼笔环肽染色检测CD99过表达对细胞形态的影响。在L428细胞中稳定转染CD99基因后，采用免疫细胞化学和流式细胞术检测分化相关蛋白表达。
CD99过表达导致L428细胞体积减小及肌动蛋白细胞骨架重排。CD99上调导致经典型霍奇金淋巴瘤诊断标志物CD30和CD15丢失，以及B细胞标志物PAX5、CD19、CD79α、BCL-6和CD10恢复。
CD99过表达导致L428细胞向B细胞再分化，提示经典型霍奇金淋巴瘤中B细胞表型的丧失很可能是CD99表达下调的结果。
