Lin X L, Sun Q C, Lu Y, Han X Q, Zhao T, Zhou X H
Department of Pathology, Southern Medical University, Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, China.
Zhonghua Xue Ye Xue Za Zhi. 2019 Jun 14;40(6):490-496. doi: 10.3760/cma.j.issn.0253-2727.2019.06.008.
To investigate the proteins expression difference after upregulation of human CD99 in Hodgkin Lymphoma cell line, L428 cell, and verify the function of differential proteins. The differential proteins were detected by two-dimensional fluorescence difference gel electrophoresis and mass spectrometry analysis, cluster analysis was done by GOfact. There were 38 proteins screened out, of which 21 proteins were positively associated with CD99, while 17 proteins were negative. Among the 38 proteins, 32 proteins participated in biological process, and 35 proteins were involved in the composition and construction. And 28 proteins participated in multifaceted biological activities including antioxidation, protein binding, catalytic activity, regulation of enzyme, signal transduction, molecular structure, regulation of translation and ion transport. The changes of the differential proteins, correlated with cytoskeleton, cell differentiation, signal pathway and regulating gene expression, are closely relevant to the translation between Hodgkin/Reed-Sternberg and B lymphocyte cell.
为研究人CD99在霍奇金淋巴瘤细胞系L428细胞中上调后蛋白质表达差异，并验证差异蛋白质的功能。采用二维荧光差异凝胶电泳和质谱分析检测差异蛋白质，通过GOfact进行聚类分析。共筛选出38种蛋白质，其中21种蛋白质与CD99呈正相关，17种蛋白质呈负相关。在这38种蛋白质中，32种蛋白质参与生物过程，35种蛋白质参与组成和构建。28种蛋白质参与多方面生物活性，包括抗氧化、蛋白质结合、催化活性、酶调节、信号转导、分子结构、翻译调节和离子转运。差异蛋白质的变化与细胞骨架、细胞分化、信号通路及调节基因表达相关，与霍奇金/里德-斯腾伯格细胞和B淋巴细胞之间的转化密切相关。
