Tian Mi, Ding Yu, Hou Deren, Deng Yanyao, Li Wei, Feng Xialu
Department of Neurology, Central South University, Changsha, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Feb;33(2):262-6.
To detect the expression of miRNA-135a-5p, miRNA-135a-2-3p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p in the brain tissue of the APPswe/PS δE9 double transgenic mouse model of Alzheimer's disease using real-time PCR.
Six-month-old APPswe/PS δE9 double transgenic mice and wild-type C57 mice of the same species were examined for the expressions of miRNA-135a-5p, miRNA-135a-2-3p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p in the brain tissue using real-time PCR.
The relative expression levels of the 5 miRNAs in the transgenic versus the wild-type mice were 0.73∓0.27 vs 1.08∓0.58, 2.47∓6.15 vs 1.65∓0.67, 0.72∓0.14 vs 1.31∓0.73, 0.57∓0.34 vs 1.06∓0.35, and 0.63∓0.26 vs 1.02∓0.18, respectively, showing significance differences in the expressions of miRNA-135a-5p, miRNA-298-5p, miRNA-466b-3p, and miR-669f-3p between the two groups (P<0.05).
miRNA-135a-5p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p are expressed differentially in APPswe/PS δE9 double transgenic mice, suggesting their important roles in the pathogenesis of Alzheimer disease.
采用实时定量聚合酶链反应检测阿尔茨海默病APPswe/PSδE9双转基因小鼠模型脑组织中miRNA-135a-5p、miRNA-135a-2-3p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p的表达。
运用实时定量聚合酶链反应检测6月龄APPswe/PSδE9双转基因小鼠和同品系野生型C57小鼠脑组织中miRNA-135a-5p、miRNA-135a-2-3p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p的表达。
5种miRNA在转基因小鼠与野生型小鼠中的相对表达水平分别为0.73±0.27对1.08±0.58、2.47±6.15对1.65±0.67、0.72±0.14对1.31±0.73、0.57±0.34对1.06±0.35、0.63±0.26对1.02±0.18，两组间miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p的表达存在显著差异（P<0.05）。
miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p在APPswe/PSδE9双转基因小鼠中表达存在差异，提示它们在阿尔茨海默病发病机制中发挥重要作用。
