Ding Yu, Tian Mi, Liu Jianfeng, Deng Yanyao, Li Wei, Feng Xialu, Hou Deren
Department of Neurology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Sep;32(9):1280-3.
To observe the changes of miRNA expression profiles in APPswe/PSδE9 transgenic mice and explore the possible roles of miRNA in the pathogenesis of Alzheimer's disease.
Using miRNA chip technique, we examined the miRNA expression in the brain tissue of 6-month-old APPswe/PSδE9 transgenic mice, with age-matched wild-type mice as the control group.
Twelve miRNAs showed differential expressions by more than two folds in APPswe/PSδE9 transgenic mice, namely miRNA-135a, miRNA-135a-2*, miRNA-298, miRNA-466b-3p, miR-669-3p, miR-142-5p, miR-144, miR-466f-3p, miR-466g, miR-200a, miR-200b and miR-96. Five miRNAs were significantly down-regulated in the transgenic mice, including miRNA-135a, miRNA-135a-2*, miRNA-298, miRNA-466b-3p, and miR-669-3p.
The 5 down- regulated miRNA may play important roles in the pathogenesis of AD in APPswe/PSδE9 transgenic mice.
观察APPswe/PSδE9转基因小鼠中微小RNA（miRNA）表达谱的变化，探讨miRNA在阿尔茨海默病发病机制中的可能作用。
采用miRNA芯片技术，检测6月龄APPswe/PSδE9转基因小鼠脑组织中的miRNA表达，以年龄匹配的野生型小鼠作为对照组。
12种miRNA在APPswe/PSδE9转基因小鼠中的表达差异超过两倍，分别为miRNA-135a、miRNA-135a-2*、miRNA-298、miRNA-466b-3p、miR-669-3p、miR-142-5p、miR-144、miR-466f-3p、miR-466g、miR-200a、miR-200b和miR-96。其中5种miRNA在转基因小鼠中显著下调，包括miRNA-135a、miRNA-135a-2*、miRNA-298、miRNA-466b-3p和miR-669-3p。
这5种下调的miRNA可能在APPswe/PSδE9转基因小鼠阿尔茨海默病的发病机制中起重要作用。
