Pisarenko O I, Studneva I M, Shul'zhenko V S, Khlopkov V N, Novikov I D, Kapel'ko V I
Biokhimiia. 1990 Jan;55(1):114-25.
The relationships between cellular energy parameters and succinate, alanine and creatine release from isolated guinea pig hearts were studied during a 50 min perfusion (0.2 ml/min) with 5.5 mM glucose or 5 mM sodium acetate. Compared to glucose-perfused hearts, a more rapid ATP depletion accompanied by an increased succinate and creatine release was observed during underperfusion with acetate. The succinate and alanine accumulation in the myocardial effluent was related to a decrease in tissue ATP; the creatine release showed a close inverse correlation with the tissue phosphocreatine/creatine ratio. Hyperbolic and linear relationships were found between these indices for glucose- and acetate-perfused hearts, respectively. The logarithm of tissue ATP had negative linear correlations with the perfusate succinate/creatine ratio for the both substrates. The experimental results suggest that succinate, creatine and alanine assays in the myocardial effluent may be used for the assessment of the energy state of ischemic heart.
在以5.5 mM葡萄糖或5 mM醋酸钠进行50分钟灌注（0.2 ml/分钟）期间，研究了分离的豚鼠心脏细胞能量参数与琥珀酸、丙氨酸和肌酸释放之间的关系。与葡萄糖灌注的心脏相比，在醋酸盐灌注不足期间观察到ATP消耗更快，同时琥珀酸和肌酸释放增加。心肌流出液中琥珀酸和丙氨酸的积累与组织ATP的减少有关；肌酸释放与组织磷酸肌酸/肌酸比值呈密切负相关。对于葡萄糖和醋酸盐灌注的心脏，分别发现这些指标之间呈双曲线和线性关系。两种底物的组织ATP对数与灌注液琥珀酸/肌酸比值呈负线性相关。实验结果表明，心肌流出液中琥珀酸、肌酸和丙氨酸的测定可用于评估缺血心脏的能量状态。
