Wong David M, Giguère Steeve, Wendel Mara A
Lloyd Veterinary Medical Center, Department of Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
J Am Vet Med Assoc. 2013 Mar 15;242(6):812-9. doi: 10.2460/javma.242.6.812.
To compare the diagnostic performance of a point-of-care (POC) analyzer with that of established methods for the measurement of plasma IgG, total protein, and albumin concentrations in neonatal foals.
Evaluation study.
100 neonatal foals < 7 days of age. Procedures-Plasma IgG, total protein, and albumin concentrations were measured with a POC analyzer via an immunoturbidimetric method. Corresponding measurements of plasma IgG, total protein, and albumin concentrations were measured by means of automated biochemical analyzers via automated immunoturbidimetric, biuret, and bromocresol green dye-binding assays, respectively (standard laboratory methods).
The sensitivity and specificity of the POC analyzer for detection of failure of passive transfer of immunity (FPTI) in foals were 80.7% and 100%, respectively, when FPTI was defined as a plasma IgG concentration < 400 mg/dL and were 75.9% and 100%, respectively, when FPTI was defined as a plasma IgG concentration < 800 mg/dL. The POC analyzer overestimated plasma albumin concentrations and, to a lesser extent, plasma total protein concentrations, compared with values determined with the standard laboratory methods.
Results suggested the POC analyzer was acceptable for determination of plasma IgG and total protein concentrations in ill foals. The POC analyzer overestimated plasma albumin concentration such that its use was clinically unacceptable for the determination of that concentration. The POC analyzer provided timely measurements of plasma IgG concentrations, which is necessary information for the assessment of passive transfer of maternal antibodies to neonatal foals.
比较即时检验（POC）分析仪与既定方法在测定新生马驹血浆IgG、总蛋白和白蛋白浓度方面的诊断性能。
评估研究。
100匹年龄小于7日龄的新生马驹。程序——通过免疫比浊法使用POC分析仪测量血浆IgG、总蛋白和白蛋白浓度。分别通过自动生化分析仪，采用自动免疫比浊法、双缩脲法和溴甲酚绿染料结合法（标准实验室方法）测量血浆IgG、总蛋白和白蛋白浓度的相应值。
当将免疫被动转移失败（FPTI）定义为血浆IgG浓度<400mg/dL时，POC分析仪检测马驹FPTI的灵敏度和特异性分别为80.7%和100%；当将FPTI定义为血浆IgG浓度<800mg/dL时，其灵敏度和特异性分别为75.9%和100%。与标准实验室方法测定的值相比，POC分析仪高估了血浆白蛋白浓度，在较小程度上也高估了血浆总蛋白浓度。
结果表明，POC分析仪可用于测定患病马驹的血浆IgG和总蛋白浓度。POC分析仪高估了血浆白蛋白浓度，因此在临床诊断该浓度时不能使用。POC分析仪能够及时测定血浆IgG浓度，这是评估母源抗体向新生马驹被动转移的必要信息。
