Osaka-sayama, Osaka, Japan From the Department of Plastic and Reconstructive Surgery, Kinki University Faculty of Medicine.
Plast Reconstr Surg. 2013 Mar;131(3):335e-342e. doi: 10.1097/PRS.0b013e31827c6dd8.
Approaches to auricular reconstruction have shown improved outcome when a basic fibroblast growth factor (bFGF) slow-release system and fibrin spraying are combined with biodegradable polymers. More complex, three-dimensional structures, such as those that replicate the human auricle, are often lost because of biodegradation of the synthetic scaffold.
To improve the mechanical strength of regenerated cartilage, the authors grafted canine autologous chondrocytes after seeding onto scaffolds made of a complex of polyglycolic acid and polypropylene, incorporating a slow-release bFGF system with a fibrin spray coating.
Five weeks after grafting, thicker cartilage with increased bending stress was obtained with the slow-release bFGF. In a three-polyglycolic acid-layer construct sandwiched around polypropylene, simulating a three-dimensional auricular structure, greater cartilage regeneration and angiogenesis were found around the implant. Sox5-positive cells were identified, indicative of maturation of neocartilage with chondroblast proliferation.
These results support the usefulness of combining absorbable and nonabsorbable materials (polyglycolic acid and polypropylene) in composite scaffolds for autologous cartilage regeneration in a large-animal autograft model.
当基本成纤维细胞生长因子（bFGF）缓释系统和纤维蛋白喷涂与可生物降解聚合物结合使用时，耳再造方法的效果得到了改善。由于合成支架的生物降解，通常会失去更复杂的三维结构，例如复制人耳的结构。
为了提高再生软骨的机械强度，作者在将犬自体软骨细胞接种到由聚乙二醇酸和聚丙烯组成的复合物制成的支架上后，加入了含有 bFGF 缓释系统的纤维蛋白喷涂涂层。
在移植后 5 周时，使用 bFGF 缓释可以获得更厚、弯曲应力更大的软骨。在由三层聚乙二醇酸夹在聚丙烯周围的三层结构中，模拟三维耳廓结构，在植入物周围发现了更多的软骨再生和血管生成。鉴定出 Sox5 阳性细胞，表明新软骨具有成软骨细胞增殖的成熟特征。
这些结果支持在大型动物自体移植物模型中，将可吸收和不可吸收材料（聚乙二醇酸和聚丙烯）结合在复合支架中用于自体软骨再生的有效性。
