Department of Gastroenterology, Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong 250014, P.R. China.
Mol Med Rep. 2013 Apr;7(4):1324-8. doi: 10.3892/mmr.2013.1340. Epub 2013 Feb 26.
Ski‑novel protein (SnoN) is a proto‑oncogene that belongs to the Ski protein family and is involved in regulating processes such as cell proliferation and apoptosis. To investigate the role of SnoN in the proliferation and apoptosis of HepG2 cells, we downregulated its expression by the use of small interfering RNA (siRNA). Three fragments predicted to have RNAi capacity were designed and synthesized as the target siRNAs (siRNA‑A, ‑B and ‑C). Following transfection, inhibition efficiency was detected by reverse transcription PCR (RT‑PCR) and western blot analysis. The siRNA with the optimal inhibition efficiency was used for the cell proliferation and apoptosis analysis. Cell proliferation was analyzed by the Cell Counting Kit‑8 (CCK‑8) and cell apoptosis was investigated by flow cytometry. In our study, all three siRNAs efficiently inhibited SnoN expression, and siRNA‑C demonstrated the optimal inhibition efficiency. We found that following downregulation of SnoN expression, HepG2 cell proliferation was significantly inhibited (P<0.05), while HepG2 cell apoptosis was significantly increased (P<0.05). SnoN‑specific siRNA is capable of effectively inhibiting the expression of SnoN in human HepG2 cells, and the downregulation of SnoN expression induces growth inhibition and apoptosis.
斯基诺蛋白（SnoN）是一种原癌基因，属于斯基蛋白家族，参与调节细胞增殖和凋亡等过程。为了研究 SnoN 在 HepG2 细胞增殖和凋亡中的作用，我们使用小干扰 RNA（siRNA）下调其表达。设计并合成了三个具有 RNAi 能力的片段作为靶 siRNA（siRNA-A、-B 和 -C）。转染后，通过逆转录 PCR（RT-PCR）和 Western blot 分析检测抑制效率。使用具有最佳抑制效率的 siRNA 进行细胞增殖和凋亡分析。通过细胞计数试剂盒-8（CCK-8）分析细胞增殖，通过流式细胞术研究细胞凋亡。在我们的研究中，所有三种 siRNA 均能有效抑制 SnoN 表达，而 siRNA-C 显示出最佳的抑制效率。我们发现，下调 SnoN 表达后，HepG2 细胞增殖明显受到抑制（P<0.05），而 HepG2 细胞凋亡明显增加（P<0.05）。SnoN 特异性 siRNA 能够有效抑制人 HepG2 细胞中 SnoN 的表达，下调 SnoN 表达诱导生长抑制和凋亡。
