Liu Chengli, Zhang Hui, Zang Xiaoxia, Wang Cheng, Kong Yalin, Zhang Hongyi
Department of Hepatobiliary Surgery, Air Force General Hospital of PLA, 30 Fucheng Road, Beijing, 100142, China.
Department of Stomatology, Air Force General Hospital of PLA, Beijing, China.
Diagn Pathol. 2015 Apr 18;10:30. doi: 10.1186/s13000-015-0267-3.
The prognosis for pancreatic cancer (PC) is very poor. The SnoN gene may have a role in cell proliferation and apoptosis in human cancer. However, the influence of SnoN on cell proliferation and apoptosis in human PC cells remains unknown.
SnoN expression was assessed in SW1990 PC cell lines using real-time polymerase chain reaction (PCR). A luciferase reporter assay was used to confirm the target associations. The effect of SnoN on cell proliferation in vitro was confirmed using Cell Counting Kit-8. Apoptosis was confirmed using flow cytometry. Gene and protein expression were examined using real time PCR and Western blotting, respectively.
SnoN siRNA significantly inhibited the growth of SW1990 cells by decreasing cell proliferation (P < 0.05) and increasing cell apoptosis (P < 0.05), compared with the blank group and the negative control group. The highest inhibition of cell proliferation appeared at 3 days post-transfection. Cell apoptosis more obvious at 48 h after transfection.
In summary, our results reveal that the RNAi-mediated downregulation of SnoN effectively inhibited the proliferation of PC cells. SnoN-siRNA also enhanced SW1990 PC cell apoptosis. These findings indicate that SnoN gene plays an important role in pancreatic cancer development, and might serve as a potential therapeutic target for pancreatic cancer. However, further in vivo studies are needed to clarify the influence of SnoN gene silencing by siRNA on pancreatic cancer therapy.
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胰腺癌（PC）的预后非常差。SnoN基因可能在人类癌症的细胞增殖和凋亡中发挥作用。然而，SnoN对人胰腺癌细胞增殖和凋亡的影响尚不清楚。
使用实时聚合酶链反应（PCR）评估SW1990胰腺癌细胞系中SnoN的表达。使用荧光素酶报告基因检测来确认靶标关联。使用细胞计数试剂盒-8确认SnoN对体外细胞增殖的影响。使用流式细胞术确认细胞凋亡。分别使用实时PCR和蛋白质印迹法检测基因和蛋白质表达。
与空白组和阴性对照组相比，SnoN siRNA通过降低细胞增殖（P < 0.05）和增加细胞凋亡（P < 0.05）显著抑制SW1990细胞的生长。转染后3天细胞增殖抑制作用最强。转染后48小时细胞凋亡更明显。
总之，我们的结果表明RNAi介导的SnoN下调有效抑制了胰腺癌细胞的增殖。SnoN-siRNA还增强了SW1990胰腺癌细胞的凋亡。这些发现表明SnoN基因在胰腺癌发展中起重要作用，可能作为胰腺癌的潜在治疗靶点。然而，需要进一步的体内研究来阐明siRNA沉默SnoN基因对胰腺癌治疗的影响。
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