Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, PO Box 217, 7500AE Enschede, The Netherlands.
Small. 2013 Oct 25;9(20):3405-9. doi: 10.1002/smll.201300220. Epub 2013 Feb 27.
Electrospun fiber meshes are patterned at length scales comparable to or lower than their fiber diameter. Simple nano- and microgrooves and closed geometric shapes are imprinted in different tones using a fast imprint process at physiological temperatures. Human mesenchymal stromal cells cultured on patterned scaffolds show differences in cellular morphology and cytoskeleton organization. Microgrooved electrospun fibers support upregulation of alkaline phosphatase and bone morphogenetic protein-2 gene expression when cells are cultured in osteogenic medium.
静电纺丝纤维网的图案设计长度尺度可与纤维直径相当或低于纤维直径。使用快速压印工艺在生理温度下，可在不同色调中压印出简单的纳米和微槽以及封闭的几何形状。在图案化支架上培养的人间质基质细胞显示出细胞形态和细胞骨架组织的差异。当细胞在成骨培养基中培养时，微槽静电纺丝纤维支持碱性磷酸酶和骨形态发生蛋白 2 基因表达的上调。
