NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, 28 Medical Drive, Singapore.
Nanomedicine (Lond). 2012 Oct;7(10):1561-75. doi: 10.2217/nnm.12.41. Epub 2012 Jun 18.
Developing 3D scaffolds mimicking the nanoscale structure of the native extracellular matrix is important in tissue regeneration. In this study, we aimed to demonstrate the novelty of 3D nanofibrous scaffolds and compare their efficiency with 2D nanofibrous scaffolds.
MATERIALS & METHODS: The 2D poly(L-lactic acid)/collagen nanofibrous scaffolds were 2D meshes fabricated by the conventional electrospinning technique, whereas the 3D poly(L-lactic acid)/collagen nanofibrous scaffolds were fabricated by a modified electrospinning technique using a dynamic liquid support system. The morphology, proliferation and differentiation abilities of human mesenchymal stem cells in osteogenic medium on both scaffolds were investigated.
RESULTS & CONCLUSION: Compared with the 2D scaffolds, the 3D scaffolds significantly increased the expression of osteoblastic genes of the stem cells as well as the formation of bone minerals. In addition, the scanning electron microscopic and micro-computed tomographic images showed the dense deposition of bone minerals aligned along the nanofibers of the 3D scaffolds after 14 and 28 days cultured with the mesenchymal stem cells. As such, the 3D electrospun poly(L-lactic acid)/collagen nanofibrous scaffold is a novel bone graft substitute for bone tissue regeneration.
开发模仿天然细胞外基质纳米结构的 3D 支架对于组织再生非常重要。本研究旨在展示 3D 纳米纤维支架的新颖性，并比较其与 2D 纳米纤维支架的效率。
2D 聚（L-乳酸）/胶原纳米纤维支架是通过传统的静电纺丝技术制备的 2D 网格，而 3D 聚（L-乳酸）/胶原纳米纤维支架是通过使用动态液体支撑系统的改良静电纺丝技术制备的。研究了成骨培养基中两种支架上的人骨髓间充质干细胞的形态、增殖和分化能力。
与 2D 支架相比，3D 支架显著增加了干细胞成骨基因的表达以及骨矿物质的形成。此外，扫描电子显微镜和微计算机断层扫描图像显示，在与间充质干细胞共培养 14 和 28 天后，3D 支架的纳米纤维上沿着纳米纤维有密集的骨矿物质沉积。因此，3D 静电纺丝聚（L-乳酸）/胶原纳米纤维支架是一种用于骨组织再生的新型骨移植替代物。
