Mahendra Jaideep, Mahendra Little, Felix John, Romanos Georgios E
Department of Periodontology, Meenakshi Ammal Dental College, Meenakshi Academy of Higher Education and Research, Chennai, India.
J Investig Clin Dent. 2014 Aug;5(3):201-7. doi: 10.1111/jicd.12030. Epub 2013 Feb 27.
The objective of the present study was to detect the presence of Porphyromonas gingivalis (fimA), Aggregatibacter actinomycetemcomitans, and red complex in the coronary plaque of patients with coronary artery disease.
The study population consisted of 51 patients with chronic periodontitis undergoing coronary artery bypass grafting. DNA was extracted from subgingival and coronary atherosclerotic plaque samples. Polymerase chain reaction was used to amplify the part of 16S rRNA gene to detect the presence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis (fimA), Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola.
Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, Porphyromonas gingivalis (fimA), and Treponema denticola were detected in 0%, 31.4%, 45.1%, 39.2%, and 51% of the atherosclerotic plaque samples, respectively. In both subgingival and coronary atherosclerotic plaque samples, Tannerella forsythia was detected in 19.6%, Porphyromonas gingivalis in 39.2%, Porphyromonas gingivalis (fimA) in 33.3%, and Treponema denticola in 35.3% of the samples.
The study confirmed the detection of red complex bacteria in coronary plaque samples. However Aggregatibacter actinomycetemcomitans could not be detected in these samples.
本研究的目的是检测冠心病患者冠状动脉斑块中牙龈卟啉单胞菌（fimA）、伴放线聚集杆菌和红色复合体的存在情况。
研究对象为51例接受冠状动脉搭桥术的慢性牙周炎患者。从龈下和冠状动脉粥样硬化斑块样本中提取DNA。采用聚合酶链反应扩增16S rRNA基因的部分片段，以检测伴放线聚集杆菌、牙龈卟啉单胞菌（fimA）、牙龈卟啉单胞菌、福赛坦纳菌和具核梭杆菌的存在情况。
在动脉粥样硬化斑块样本中，伴放线聚集杆菌、福赛坦纳菌、牙龈卟啉单胞菌、牙龈卟啉单胞菌（fimA）和具核梭杆菌的检出率分别为0%、31.4%、45.1%、39.2%和51%。在龈下和冠状动脉粥样硬化斑块样本中，福赛坦纳菌在19.6%的样本中被检测到，牙龈卟啉单胞菌在39.2%的样本中被检测到，牙龈卟啉单胞菌（fimA）在33.3%的样本中被检测到，具核梭杆菌在35.3%的样本中被检测到。
该研究证实了在冠状动脉斑块样本中检测到红色复合体细菌。然而，在这些样本中未检测到伴放线聚集杆菌。
