Schulz Susanne, Hofmann Britt, Grollmitz Julia, Friebe Lisa, Kohnert Michael, Schaller Hans-Günter, Reichert Stefan
Department of Operative Dentistry and Periodontology, Martin-Luther-University Halle-Wittenberg, 06112 Halle, Germany.
Department of Cardiothoracic Surgery, Heart Centre of the University Clinics Halle (Saale), Martin-Luther-University Halle-Wittenberg, 06112 Halle, Germany.
Biomedicines. 2022 Jul 27;10(8):1801. doi: 10.3390/biomedicines10081801.
The oral microbiota has been implicated in a variety of systemic diseases, including cardiovascular (CV) disease. The main objective of this study (DRKS-ID: DRKS00015776) was to evaluate the prognostic importance of the oral microbiota for further CV events in patients undergoing coronary artery bypass grafting surgery (3-year follow-up).
In this longitudinal cohort study, 102 CV patients were enrolled, of whom 95 completed the 3-year follow-up. The CV outcome was assessed using the major adverse cardiac and cerebrovascular events criteria. To evaluate subgingival colonization, 16S rRNA genes were amplified, targeting the V3/V4 region (Illumina MiSeq).
Regarding the specific number of operational taxonomic units (OTUs), no significant differences in CV outcome were determined (alpha diversity, Shannon index). In linear discriminant analyses and -tests, the disease-specific differences in the beta diversity of the microbiota composition were evaluated. It was evident that bacteria species of the genus were significantly more prevalent in patients with a secondary CV event ( = 0.015). This hierarchical order also includes , which is considered to be of comprehensive importance in both periodontal and CV diseases.
Here, we proved that subgingival occurrence of species has prognostic relevance for cardiovascular outcomes in CV patients undergoing coronary artery bypass grafting.
口腔微生物群与多种全身性疾病有关，包括心血管（CV）疾病。本研究（DRKS-ID：DRKS00015776）的主要目的是评估口腔微生物群对接受冠状动脉搭桥手术患者（3年随访）进一步发生CV事件的预后重要性。
在这项纵向队列研究中，招募了102名CV患者，其中95名完成了3年随访。使用主要不良心脑血管事件标准评估CV结局。为了评估龈下定植情况，扩增16S rRNA基因，靶向V3/V4区域（Illumina MiSeq）。
关于可操作分类单元（OTU）的具体数量，未确定CV结局有显著差异（α多样性，香农指数）。在线性判别分析和检验中，评估了微生物群组成的β多样性的疾病特异性差异。很明显，继发CV事件的患者中属的细菌种类明显更普遍（=0.015）。这个层级顺序还包括，其在牙周病和CV疾病中都被认为具有综合重要性。
在此，我们证明了属物种的龈下出现对接受冠状动脉搭桥手术的CV患者的心血管结局具有预后相关性。
