Jin Hai-Xia, Xin Zhi-Min, Song Wen-Yan, Dai Shan-Jun, Sun Ying-Pu
Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
J Reprod Med. 2013 Jan-Feb;58(1-2):51-4.
To observe the effects of cumulus cells on in vitro fertilization.
Oocytes were retrieved from 47 patients (> 10/patient) who underwent short-term insemination from August 2009 to June 2010. The oocytes from each patient were divided into a cumulus cell-free group (cumulus cells were removed from the incubation medium 4 hours after coincubation of male and female gametes) with 389 oocytes and a cumulus cell group (cumulus cells were retained with the gametes until fertilization was evaluated 16-18 hours after co-incubation) with 402 oocytes.
Polyspermic fertilization was 0.96 +/- 1.14 in the cumulus cell-free group and 0.47 +/- 0.72 in the cumulus cell group with p < 0.05. There were no significant differences in normal fertilization (5.96 ğ 1.73 vs. 6.55 +/- 3.72), 1PN fertilization (0.06 +/- 0.25 vs. 0.09 +/- 0.28), fertilization failure (1.34 +/- 1.17 vs. 1.45 +/- 1.84), cleavage (6.06 +/- 2.04 vs. 6.51 +/- 3.94), high-quality embryo (3.94 +/- 1.79 vs. 4.74 +/- 3.45) and usable embryo (5.06 +/- 1.86 vs. 5.68 +/- 3.98) between cumulus cell-free group and cumulus cell group, all with p > 0.05.
In our study short-term insemination (4 hours) causes a statistical increase in polyspermic fertilization. In order to ensure correct oocyte fertilization and reduction of polyspermic fertilization, it is better to retain the cumulus cells for 16-18 hours.
观察卵丘细胞对体外受精的影响。
从2009年8月至2010年6月接受短期授精的47例患者（每位患者超过10个）中获取卵母细胞。将每位患者的卵母细胞分为无卵丘细胞组（雌雄配子共孵育4小时后从培养液中去除卵丘细胞），共389个卵母细胞，以及卵丘细胞组（卵丘细胞与配子一起保留，直到共孵育16 - 18小时后评估受精情况），共402个卵母细胞。
无卵丘细胞组的多精受精率为0.96±1.14，卵丘细胞组为0.47±0.72，p<0.05。无卵丘细胞组与卵丘细胞组在正常受精率（5.96±1.73对6.55±3.72）、1PN受精率（0.06±0.25对0.09±0.28）、受精失败率（1.34±1.17对1.45±1.84）、卵裂率（6.06±2.04对6.51±3.94）、优质胚胎率（3.94±1.79对4.74±3.45）和可用胚胎率（5.06±1.86对5.68±3.98）方面均无显著差异，p均>0.05。
在我们的研究中，短期授精（4小时）导致多精受精在统计学上增加。为确保卵母细胞正确受精并减少多精受精，最好将卵丘细胞保留16 - 18小时。
