关于具有机械加工表面和粗糙表面的钛植入物周围上皮细胞行为的体内和体外研究。
In vivo and in vitro studies of epithelial cell behavior around titanium implants with machined and rough surfaces.
作者信息
Atsuta Ikiru, Ayukawa Yasunori, Furuhashi Akihiro, Ogino Yoichiro, Moriyama Yasuko, Tsukiyama Yoshihiro, Koyano Kiyoshi
机构信息
Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan; Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, Los Angeles, California, USA.
出版信息
Clin Implant Dent Relat Res. 2014 Oct;16(5):772-81. doi: 10.1111/cid.12043. Epub 2013 Feb 28.
BACKGROUND
The surface roughness of a dental implant affects the epithelial wound healing process and may significantly enhance implant prognosis.
PURPOSE
We explored the influence of surface roughness on peri-implant epithelium (PIE) sealing and down-growth by comparing machine-surfaced (Ms) and rough-surfaced (Rs) implants.
MATERIALS AND METHODS
(1) Maxillary first molars were extracted from rats and replaced with Ms or Rs implants. (2) We also compared changes in the morphology of cultured rat oral epithelial cells (OECs) grown on Ms or Rs titanium (Ti) plates.
RESULTS
(1) After 4 weeks, the PIE around Ms and Rs implants showed a similar structure to junctional epithelium (JE). At 16 weeks, Rs implants appeared to form a weak epithelial seal at the tissue-implant interface and exhibited markedly less PIE down-growth than Ms implants but was deeper than that observed in natural teeth. (2) We observed less expression of adhesion proteins in OECs cultured on Rs plates than in cells grown on Ms plates. Additionally, cell adherence, migration, and proliferation on Rs plates were lower, whereas apoptosis was reduced on Ms plates.
CONCLUSION
Ms implants are a better choice for integration with an epithelial wound healing process.
背景
牙种植体的表面粗糙度会影响上皮伤口愈合过程，并可能显著改善种植体预后。
目的
通过比较机械加工表面（Ms）和粗糙表面（Rs）种植体，探讨表面粗糙度对种植体周围上皮（PIE）封闭和向下生长的影响。
材料与方法
（1）从大鼠体内拔除上颌第一磨牙，并用Ms或Rs种植体进行替换。（2）我们还比较了在Ms或Rs钛（Ti）板上培养的大鼠口腔上皮细胞（OECs）形态的变化。
结果
（1）4周后，Ms和Rs种植体周围的PIE显示出与结合上皮（JE）相似的结构。在16周时，Rs种植体在组织-种植体界面似乎形成了薄弱的上皮封闭，并且PIE向下生长明显少于Ms种植体，但比天然牙中观察到的更深。（2）我们观察到，在Rs板上培养的OECs中黏附蛋白的表达低于在Ms板上生长的细胞。此外，Rs板上的细胞黏附、迁移和增殖较低，而Ms板上的细胞凋亡减少。
结论
Ms种植体是与上皮伤口愈合过程整合的更好选择。
Zotero 插件