Institute for Genetic Engineering and Biotechnology Sarajevo, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and Herzegovina.
Bosn J Basic Med Sci. 2013 Feb;13(1):10-3. doi: 10.17305/bjbms.2013.2403.
There are two major theories for inheritance of Rh blood group system: Fisher - Race theory and Wiener theory. Aim of this study was identifying frequency of RHDCE alleles in Bosnian - Herzegovinian population and introduction of this method in screening for Rh phenotype in B&H since this type of analysis was not used for blood typing in B&H before. Rh blood group was typed by Polymerase Chain Reaction, using the protocols and primers previously established by other authors, then carrying out electrophoresis in 2-3% agarose gel. Percentage of Rh positive individuals in our sample is 84.48%, while the percentage of Rh negative individuals is 15.52%. Inter-rater agreement statistic showed perfect agreement (K=1) between the results of Rh blood system detection based on serological and molecular-genetics methods. In conclusion, molecular - genetic methods are suitable for prenatal genotyping and specific cases while standard serological method is suitable for high-throughput of samples.
关于 Rh 血型系统遗传有两种主要理论：Fisher-Race 理论和 Wiener 理论。本研究的目的是确定波黑人群中 RHDCE 等位基因的频率，并将这种方法引入波黑的 Rh 表型筛选中，因为在此之前，这种类型的分析并未用于波黑的血型鉴定。Rh 血型通过聚合酶链反应进行分型，使用先前由其他作者建立的方案和引物，然后在 2-3%琼脂糖凝胶中进行电泳。我们样本中 Rh 阳性个体的百分比为 84.48%，而 Rh 阴性个体的百分比为 15.52%。观察者间一致性统计显示，基于血清学和分子遗传学方法的 Rh 血型系统检测结果之间存在完全一致（K=1）。总之，分子遗传学方法适用于产前基因分型和特定情况，而标准血清学方法适用于高通量样本。
