Matsumoto Teruki, Takebayashi Jun, Ishimi Yoshiko, Ozawa Chihiro, Sano Akira, Hirota Takashi, Endoh Kaori
National Institute of Health and Nutrition, Department of Food Function and Labeling, 1-23-1, Toyama, Shinjuku, Tokyo 162-8636, Japan.
J AOAC Int. 2012 Nov-Dec;95(6):1609-13. doi: 10.5740/jaoacint.11-417.
As a part of the development of an alternative to microbiological assay for vitamin B12, we performed a quantitative analysis of cyanocobalamin (CN-cbl) in a National Institute of Standards and Technology Standard Reference Material (SRM 3280) by HPLC. Using this method, the observed value (4.64 microg/g) of CN-cbl in SRM 3280 was found to be in good agreement with the certified value (4.80 microg/g). The accuracy was over 95%, with a corresponding measurement precision value of 5%. To evaluate the applicability of the method on commercial multivitamin tablets, the method was applied to a variety of these samples. The present method has a good accuracy and precision to evaluate CN-cbl with respect to all of the examined tablets.
作为开发维生素B12微生物测定替代方法的一部分，我们通过高效液相色谱法（HPLC）对国家标准与技术研究院标准参考物质（SRM 3280）中的氰钴胺（CN-cbl）进行了定量分析。采用该方法，发现SRM 3280中CN-cbl的观测值（4.64微克/克）与认证值（4.80微克/克）高度一致。准确度超过95%，相应的测量精密度值为5%。为评估该方法对市售多种维生素片剂的适用性，将该方法应用于多种此类样品。就所有检测的片剂而言，本方法在评估CN-cbl方面具有良好的准确度和精密度。
