Jiangsu Key Laboratory for Biodiversity & Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China.
Fish Shellfish Immunol. 2013 May;34(5):1287-93. doi: 10.1016/j.fsi.2013.02.014. Epub 2013 Feb 27.
Toll receptor was first discovered in Drosophila and has an important function in the innate immunity of invertebrates. In this study, the Toll receptor HcToll1 from Hyriopsis cumingii with a full length of 3810 bp consisting of a 3687 bp ORF that encodes a total of 1228 amino acids protein was selected for further study. The HcToll1 protein consisted of a signal peptide, 17 LRR domains, 2 LRRCT domains, 1 LRRNT domain, 1 TM domain, and 1 TIR domain. Phylogenetic analysis results showed that HcToll1 was clustered in one group together with other mollusca tolls. RT-PCR analysis results showed that HcToll1 was expressed in all tested tissues such as hemocytes, hepatopancreas, gills, and mantle. qRT-PCR analysis results showed that HcToll1 expression was increased by the presence of Escherichia coli, Vibrio anguillarum, Staphyloccocus aureus, and White Spot Syndrome Virus (WSSV). Over-expression of HcTIR could up-regulate expression of drosomycin gene in Drosophila S2 cells. The results of our study indicated that HcToll1 is a functional Toll and it has an important function in the generation of innate immune responses of H. cumingii against microbial challenge.
Toll 受体最初在果蝇中被发现，在无脊椎动物的先天免疫中具有重要功能。在本研究中，选择长 3810bp 的中华绒螯蟹 Toll 受体 HcToll1 进行进一步研究，其全长 3687bp 的 ORF 编码一个共 1228 个氨基酸的蛋白质。HcToll1 蛋白由信号肽、17 个 LRR 结构域、2 个 LRRCT 结构域、1 个 LRRNT 结构域、1 个 TM 结构域和 1 个 TIR 结构域组成。系统进化分析结果表明，HcToll1 与其他软体动物 tolls 聚为一簇。RT-PCR 分析结果表明，HcToll1 在所有检测组织中均有表达，如血细胞、肝胰腺、鳃和套膜。qRT-PCR 分析结果表明，Escherichia coli、Vibrio anguillarum、Staphyloccocus aureus 和 White Spot Syndrome Virus (WSSV) 的存在均可诱导 HcToll1 的表达。HcTIR 的过表达可上调果蝇 S2 细胞中 drosomycin 基因的表达。本研究结果表明，HcToll1 是一种功能性 Toll，在中华绒螯蟹抵御微生物挑战产生先天免疫反应中具有重要功能。
