伊氏锥虫：使用 TBR(1) 和 TBR(2) 引物检测自然和实验感染动物中的伊氏锥虫 DNA。
Trypanosoma evansi: detection of Trypanosoma evansi DNA in naturally and experimentally infected animals using TBR(1) & TBR(2) primers.
机构信息
Zoology Department, Faculty of Science, Ain Shams University, P.O. Box 11566, Cairo, Egypt.
出版信息
Exp Parasitol. 2013 May;134(1):109-14. doi: 10.1016/j.exppara.2013.02.003. Epub 2013 Feb 27.
A polymerase chain reaction (PCR-based) assay was evaluated for detection of Trypanosoma evansi DNA in experimentally infected mice and naturally infected camels, sheep and goats using the set of primers TBr(1) & TBr(2) that amplified 164 bp DNA fragment. The results revealed that PCR-based assay was able to detect T. evansi directly from the blood during both acute and chronic phase of infection in all tested animals and in the blood and tissues of intraperitoneally infected mice depending upon the level of infection in the test samples. PCR was more powerful than CATT/T. evansi and mouse inoculation tests, when detected the infection in mice (24 h) post infection. Present results show that sheep & goats probably play a role in transmission of T. evansi to camels and supported that PCR could be used as a diagnostic tool for epidemiological studies on T. evansi in Egypt.
聚合酶链反应（PCR 为基础）检测法用于检测实验组感染的小鼠和自然感染的骆驼、绵羊和山羊中的伊氏锥虫 evansi DNA，使用引物 TBr(1) 和 TBr(2) 扩增 164 bp 的 DNA 片段。结果表明，该 PCR 检测法能够在所有受测动物的急性和慢性感染期直接从血液中检测到 T. evansi，也能够在经腹腔感染的小鼠的血液和组织中检测到，这取决于测试样本中的感染程度。与 CATT/T. evansi 和小鼠接种试验相比，PCR 能够在感染后 24 小时检测到小鼠的感染，更为有效。目前的结果表明，绵羊和山羊可能在将 T. evansi 传播给骆驼方面发挥作用，并支持 PCR 可作为埃及 T. evansi 流行病学研究的一种诊断工具。
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