比较血清学和分子检测方法在阿尔及利亚南部加尔德地区家畜中检测伊氏锥虫的效果。
Comparison of serological and molecular tests for detection of Trypanosoma evansi in domestic animals from Ghardaïa district, South Algeria.
机构信息
High National Veterinary School of Algiers, Issad Street, Oued Smar, Bab Ezzouar, Algeria; Akli Mohand Oulhadj University, Department of Agriculture Science, Drissi Yahia Street, Bouira, Algeria.
Institute of Tropical Medicine, Department of Biomedical Sciences, Nationalestraat 155, 2000 Antwerp, Belgium.
出版信息
Vet Parasitol. 2020 Apr;280:109089. doi: 10.1016/j.vetpar.2020.109089. Epub 2020 Mar 19.
Trypanosoma evansi (T. evansi) is a hemoflagellate parasite that affects a broad range of mammalian hosts and that causes a disease called surra. Diagnosis of surra based on clinical symptoms alone is inaccurate. Therefore, a variety of serological and molecular diagnostic tests are used to assist in the detection of T. evansi infections. The aim of this study was to compare the diagnostic performance of four serological tests (CATT/T.evansi, immune trypanolysis, ELISA with purified variant surface glycoprotein RoTat 1.2 and with whole cell lysate) and two molecular PCR tests targeting sequences within the ribosomal genes locus (ITS1 TD PCR and 18S qPCR). Tests were carried out on blood samples from 161 dromedary camels, 93 horses, 129 goats, 168 sheep, 127 bovines and 76 dogs. Latent class analysis was carried out to calculate the sensitivity and specificity of each diagnostic test. Cohen's Kappa test was used to assess the concordance between the different diagnostic tests. Overall positivity rates observed with the serological tests were as follows: 3.1 % with CATT/T.evansi, 4.9 % with ELISA/RoTat 1.2, 3.4 % with ELISA/whole lysate and 2.0 % with immune trypanolysis (TL). Among the 754 samples tested with the molecular tests, 1.7 % were positive with 18S qPCR and 1.3 % with ITS1 TD PCR. Cohen's Kappa test showed agreement ranging from fair to substantial (k = 0.2-0.8) between serological diagnostic tests. However, it showed a perfect agreement (k = 0.868) between molecular diagnostic tests. Latent class analysis showed that all serological tests were 100 % sensitive, in contrast to the molecular tests with 47 % sensitivity. All tests, though, were highly specific (≥ 97 %). Given the persistence of circulating antibodies after cure, detectable by serological tests, it is recommend combining a serological and a molecular diagnostic test for accurate diagnosis of infection with T. evansi in domestic animals.
伊氏锥虫（T. evansi）是一种血鞭毛原虫寄生虫，可感染多种哺乳动物宿主，并导致苏拉病。仅基于临床症状诊断苏拉病并不准确。因此，使用了各种血清学和分子诊断检测方法来协助检测 T. evansi 感染。本研究的目的是比较四种血清学检测方法（CATT/T.evansi、免疫溶血、使用纯化的变体表面糖蛋白 RoTat 1.2 和全细胞裂解物的 ELISA 以及针对核糖体基因座内序列的两种分子 PCR 检测方法（ITS1 TD PCR 和 18S qPCR）的诊断性能。对 161 只单峰驼、93 匹马、129 只山羊、168 只绵羊、127 头牛和 76 只狗的血液样本进行了检测。进行了潜在类别分析，以计算每种诊断测试的敏感性和特异性。Kappa 检验用于评估不同诊断测试之间的一致性。使用血清学检测方法观察到的总体阳性率如下：CATT/T.evansi 为 3.1%、ELISA/RoTat 1.2 为 4.9%、ELISA/whole lysate 为 3.4%和免疫溶血（TL）为 2.0%。在使用分子检测方法检测的 754 个样本中，18S qPCR 为 1.7%，ITS1 TD PCR 为 1.3%。Kappa 检验显示血清学诊断检测之间的一致性从适度到高度（k=0.2-0.8）。然而，它显示出完美的一致性（k=0.868），分子诊断检测之间的一致性。潜在类别分析显示，所有血清学检测的敏感性均为 100%，而分子检测的敏感性为 47%。所有检测方法的特异性均较高（≥97%）。鉴于治愈后可检测到循环抗体，因此建议将血清学和分子诊断检测相结合，以准确诊断家畜感染伊氏锥虫。
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