Yashima Y, McAuliffe D J, Flotte T J
Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Boston 02114.
Lasers Surg Med. 1990;10(3):280-3. doi: 10.1002/lsm.1900100308.
Cell selectivity to photoacoustic injury induced by argon-fluoride excimer laser (193 nm) was studied. Rats were irradiated through air or water and a 2.5 mm aperture. The laser was adjusted to deliver 150 mJ/cm2 at the skin surface with 12 and 24 pulses. Immediate damage was assessed by transmission electron microscopy. Cell selectivity was observed in dermis and epidermis. Fibroblasts showed alteration of nuclear chromatin and cytoplasmic organelles, while some of the migratory cells adjacent to fibroblasts did not. Similar difference of damage was observed between keratinocytes and Langerhans cells in epidermis. Considering the relationship between cells and their microenvironment in tissue, this selectivity may be due to the difference of acoustical coupling of propagation of acoustic waves rather than to differential sensitivity of the cells to damage.
研究了细胞对氟化氩准分子激光（193nm）诱导的光声损伤的选择性。通过空气或水以及一个2.5mm的孔径对大鼠进行照射。将激光调整为在皮肤表面以12次和24次脉冲传递150mJ/cm²的能量。通过透射电子显微镜评估即时损伤。在真皮和表皮中观察到细胞选择性。成纤维细胞显示出核染色质和细胞质细胞器的改变，而与成纤维细胞相邻的一些迁移细胞则没有。在表皮的角质形成细胞和朗格汉斯细胞之间也观察到类似的损伤差异。考虑到组织中细胞与其微环境之间的关系，这种选择性可能是由于声波传播的声学耦合差异，而不是细胞对损伤的不同敏感性。
