Hajari Taheri Fatemeh, Seyedolmohadesin Maryam, Bayat Mansour, Mahdavi Mehdi, Yazdi Mohammad Hossein, Eslamifar Ali, Abolhasani Mohsen
Department of Immunology, Hybridoma Lab, Pasteur Institute of Iran, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2013 Mar;12(1):81-5.
Breast cancer patients are susceptible to infections such as candidiasis. Due to the importance and the role of matrix metalloproteinases (MMP) in breast cancer progression and its correlation with tumor metastasis, we analyzed the serum level of MMPs -2, -3, -9 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in breast cancer bearing mice in the presence of systemic Candida albicans infection. Female BALB/c mice were divided into 4 groups: group I had tumor + candidiasis; group II, tumor only; group III, candidiasis only and group IV as negative control. Tumor tissue was separated from stock breast cancer bearing mice and transplanted subcutaneously into the groups I and II mice. Two weeks after tumor transplantation, groups I and III were infected with Candida albicans by intravenous injection. One week after systemic infection, the sera of the experimental groups were prepared and analyzed with ELISA for MMP-2, -3, -9 and TIMP-1 levels. The results showed that the levels of MMP-3, MMP-9 and TIMP-1 were increased in groups I, II and III, as compared to the control group. However, the level of MMP-2 was decreased in mice infected with Candida albicans and in infected mice bearing tumor. These data suggest that candidiasis may have a positive effect on tumor progression and metastasis.
乳腺癌患者易感染念珠菌病等感染。由于基质金属蛋白酶（MMP）在乳腺癌进展中的重要性和作用及其与肿瘤转移的相关性，我们分析了全身性白色念珠菌感染情况下荷乳腺癌小鼠血清中MMP -2、-3、-9以及基质金属蛋白酶组织抑制剂-1（TIMP-1）的水平。雌性BALB/c小鼠分为4组：第I组为肿瘤+念珠菌病组；第II组为仅肿瘤组；第III组为仅念珠菌病组；第IV组为阴性对照组。从荷乳腺癌的种鼠分离肿瘤组织并皮下移植到第I组和第II组小鼠体内。肿瘤移植两周后，第I组和第III组通过静脉注射感染白色念珠菌。全身感染一周后，制备实验组血清并用ELISA分析MMP-2、-3、-9和TIMP-1水平。结果显示，与对照组相比，第I、II和III组中MMP-3、MMP-9和TIMP-1水平升高。然而，感染白色念珠菌的小鼠以及荷瘤感染小鼠中MMP-2水平降低。这些数据表明念珠菌病可能对肿瘤进展和转移有积极影响。
