Sengupta Mandar, Chakraborty Anindita, Raychaudhuri Sarmistha Sen
Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, APC Road, Kolkata-700009, India.
Appl Radiat Isot. 2013 May;75:44-9. doi: 10.1016/j.apradiso.2013.01.036. Epub 2013 Feb 6.
Effects of gamma rays on the free polyamine (PA) levels were studied in Vigna radiata (L.) Wilczek. Seeds exposed to different doses of gamma rays were checked for damage on phenotype, germination frequency and alteration in photosynthetic pigments. Free polyamine levels were estimated from seeds irradiated in dry and water imbibed conditions. Polyamine levels of seedlings grown from irradiated seeds, and irradiated seedlings from unexposed seeds were also measured. Damage caused by gamma irradiation resulted in decrease in final germination percentage and seedling height. Photosynthetic pigments decreased in a dose dependent manner as marker of stress. Polyamines decreased in irradiated dry seeds and in seedlings grown from irradiated seeds. Radiation stress induced increase in free polyamines was seen in irradiated imbibed seeds and irradiated seedlings. Response of polyamines towards gamma rays is dependent on the stage of the life cycle of the plant.
研究了γ射线对绿豆（Vigna radiata (L.) Wilczek）游离多胺（PA）水平的影响。检测了暴露于不同剂量γ射线的种子在表型、发芽频率以及光合色素变化方面的损伤情况。对在干燥和吸水条件下辐照的种子进行游离多胺水平评估。还测定了由辐照种子生长而来的幼苗以及从未经辐照种子辐照而来的幼苗的多胺水平。γ辐射造成的损伤导致最终发芽率和幼苗高度降低。光合色素作为胁迫指标呈剂量依赖性降低。辐照干燥种子以及由辐照种子生长而来的幼苗中的多胺减少。在辐照吸水种子和辐照幼苗中观察到辐射胁迫诱导游离多胺增加。多胺对γ射线的响应取决于植物生命周期的阶段。
